Tarfeen Najeebul, Ul Nisa Khair, Masoodi Shariq Rashid, Bhat Humaira, Wani Saba, Ganai Bashir Ahmad
Centre of Research for Development, University of Kashmir, Srinagar, Jammu and Kashmir 190006 India.
Department of Environmental Science, University of Kashmir, Srinagar, Jammu and Kashmir 190006 India.
Indian J Clin Biochem. 2024 Oct;39(4):586-592. doi: 10.1007/s12291-023-01144-1. Epub 2023 Jul 28.
In this study, the role of inflammatory biomarkers and vitamin D in Type 2 diabetes mellitus (T2DM) and their correlation with diabetes related factors (HbA1c, FPG, and insulin) was analysed. In this study, Kashmiri patients with T2DM and healthy individuals were considered as cases (n = 100) and controls (n = 100) respectively. Blood samples from both groups were collected, inflammatory biomarkers (TNF-α, CRP), as well as serum vitamin D levels, were estimated by ELISA. From our results it was revealed that patients with T2DM had significantly lower serum vitamin D levels than control groups (<0.05). Pro-inflammatory cytokine levels, including TNF-α and CRP, were seen to be elevated reaching a level of statistical significance (<0.05). On correlating the HbA1c, FPG and insulin with TNF-α, CRP and vitamin D, significant positive correlation (<0.05) was found between TNF-α and CRP with HbA1c and FPG in patients, non-significant positive correlation (>0.05) was observed between insulin with TNF-α, and vitamin D and weak negative correlation with CRP in case study group. On correlating the impact of vitamin D on HbA1c and FPG levels, non-significant weak negative correlation was observed in patient group than controls, indicating that patients with lower vitamin D levels have higher HbA1c, showing that lower vitamin D have some role in etiology of T2DM.
在本研究中，分析了炎症生物标志物和维生素D在2型糖尿病（T2DM）中的作用及其与糖尿病相关因素（糖化血红蛋白、空腹血糖和胰岛素）的相关性。在本研究中，克什米尔T2DM患者和健康个体分别被视为病例组（n = 100）和对照组（n = 100）。采集两组的血样，通过酶联免疫吸附测定法（ELISA）测定炎症生物标志物（肿瘤坏死因子-α、C反应蛋白）以及血清维生素D水平。我们的结果显示，T2DM患者的血清维生素D水平显著低于对照组（<0.05）。促炎细胞因子水平，包括肿瘤坏死因子-α和C反应蛋白，被发现有所升高，达到统计学显著水平（<0.05）。在将糖化血红蛋白、空腹血糖和胰岛素与肿瘤坏死因子-α、C反应蛋白和维生素D进行相关性分析时，发现患者中肿瘤坏死因子-α和C反应蛋白与糖化血红蛋白和空腹血糖之间存在显著正相关（<0.05），在病例研究组中，胰岛素与肿瘤坏死因子-α、维生素D之间观察到非显著正相关（>0.05），与C反应蛋白呈弱负相关。在分析维生素D对糖化血红蛋白和空腹血糖水平的影响时，患者组与对照组相比观察到非显著弱负相关，这表明维生素D水平较低的患者糖化血红蛋白水平较高，表明较低的维生素D水平在T2DM的病因中起到一定作用。
