Gadalla Dina, Kennedy Maeve M, Lott David G
Head and Neck Regenerative Medicine Laboratory, Mayo Clinic, Phoenix, AZ, USA.
Division of Laryngology, Mayo Clinic, Phoenix, AZ, USA.
Bio Protoc. 2024 Sep 20;14(18):e4974. doi: 10.21769/BioProtoc.4974.
Tissue-engineered constructs combine the mechanical properties of biomaterials with biological agents to serve as scaffolds that direct the wound-healing process and promote tissue regeneration. A limitation to studying wound healing in vivo is that mouse skin contracts to heal rather than exhibiting granulation tissue formation and epithelialization like human skin. Therefore, it became necessary to develop a mouse model to better recapitulate human wound healing. The first splinted excisional wound healing model in mice, described in 2004, utilized silicone splints to prevent skin contracture.This model has been used to test a variety of wound healing strategies; however, to our knowledge, this model has not been adapted to test the effect of implants on wound healing. In our established protocol, circular bilateral excisional wounds are made on the mouse's dorsum. A circular implant made of porous polyethylene is sutured to the skin within the wound. A thin, donut-shaped silicone splint is secured to the skin surrounding the wound, and a thick, donut-shaped splint is placed on top to tent the wound dressing. Finally, the mouse's abdomen is wrapped in a bandage and tape toprotect the implants. Our protocol offers a significant enhancement to the existing model by enabling the testing of implants for wound healing, as well as using an additional splint that prevents direct contact between the wound dressing and the wound bed. This model can be used to study tissue-engineered implant designs in a relatively low-cost, simple, and high-throughput manner before advancing to larger animal studies. Key features • Builds up on methods developed by Galiano et al. [1] and extends their application to include scaffold testing. • Utilizes a construct that protects wounds, thereby enabling unaffected wound healing. • Can be adapted to test a wide variety of biomaterials for wound healing. • Describes dressing details and exact methodologies that prevent animals from interfering with wound healing.
组织工程构建体将生物材料的机械性能与生物因子相结合，作为引导伤口愈合过程和促进组织再生的支架。在体内研究伤口愈合的一个局限性在于，小鼠皮肤通过收缩来愈合，而不像人类皮肤那样表现出肉芽组织形成和上皮化。因此，有必要开发一种小鼠模型，以更好地模拟人类伤口愈合过程。2004年描述的首个小鼠夹板切除伤口愈合模型，利用硅胶夹板防止皮肤挛缩。该模型已被用于测试多种伤口愈合策略；然而，据我们所知，该模型尚未被用于测试植入物对伤口愈合的影响。在我们既定的方案中，在小鼠背部制造圆形双侧切除伤口。将由多孔聚乙烯制成的圆形植入物缝合到伤口内的皮肤上。一个薄的、甜甜圈形状的硅胶夹板固定在伤口周围的皮肤上，一个厚的、甜甜圈形状的夹板放在上面撑起伤口敷料。最后，用绷带和胶带包裹小鼠腹部以保护植入物。我们的方案对现有模型有显著改进，能够测试用于伤口愈合的植入物，以及使用额外的夹板防止伤口敷料与伤口床直接接触。该模型可用于以相对低成本、简单且高通量的方式研究组织工程植入物设计，然后再推进到更大动物的研究。关键特性 • 基于Galiano等人[1]开发的方法，并将其应用扩展到包括支架测试。 • 使用一种保护伤口的构建体，从而实现不受影响的伤口愈合。 • 可适用于测试多种用于伤口愈合的生物材料。 • 描述了防止动物干扰伤口愈合的敷料细节和确切方法。
