Ren Licheng, Zhou Bo, Chen Lei
Xiangya Hospital, Central South University, Changsha, Hunan, China; email:
Xiangya Hospital, Central South University, Changsha, Hunan, China.
Wounds. 2012 Feb;24(2):36-42.
A good animal model for wound healing is indispensable for researchers to study the basic mechanism of tissue repair, and to develop strategies for clinical treatment. Small mammalian wound healing models are the most popular animal models for wound healing research because they are inexpensive, readily obtainable, and easy to handle. One significant challenge of using mice to evaluate wound repair is that wound contraction originates outside of tissue, whereas in humans, re-epithelialization and granulation tissue formation occurs within the wound space.
The present study describes a new excisional skin wound model utilizing an implanted silicone ring on the dorsal side of the mouse for 1 week prior to creating a fullthickness skin defect wound.
The results showed that the time required for complete epithelialization of the wound was extended, the re epithelialization ratio was increased, and more granulation tissue was formed.
Permitting the wound to heal mainly through re-epithelialization and granulation tissue formation, this new technique can result in a new excisional murine wound model that closer approximates human wound healing, allowing for more relevant evaluation of molecular signaling and cellular metabolism that occur during skin wound healing. .
对于研究人员而言，一个良好的伤口愈合动物模型对于研究组织修复的基本机制以及开发临床治疗策略是不可或缺的。小型哺乳动物伤口愈合模型是伤口愈合研究中最常用的动物模型，因为它们成本低、容易获得且易于操作。使用小鼠评估伤口修复的一个重大挑战在于伤口收缩起源于组织外部，而在人类中，伤口空间内会发生再上皮化和肉芽组织形成。
本研究描述了一种新的切除性皮肤伤口模型，即在创建全层皮肤缺损伤口前1周，在小鼠背部植入硅胶环。
结果显示，伤口完全上皮化所需时间延长，再上皮化率增加，并且形成了更多的肉芽组织。
这种新技术允许伤口主要通过再上皮化和肉芽组织形成来愈合，能够产生一种更接近人类伤口愈合的新的切除性小鼠伤口模型，从而可以对皮肤伤口愈合过程中发生的分子信号传导和细胞代谢进行更相关的评估。
