Laboratory of Tissue Repair and Regeneration, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Methods Mol Biol. 2021;2299:405-417. doi: 10.1007/978-1-0716-1382-5_27.
Human hypertrophic scars are the result of imperfect healing of skin, which is particularly evident from the scars developing after severe burns. In contrast, mouse and rat full-thickness skin wounds heal normally without forming visible scar tissue, which reduces the suitability of rodent models for the study of skin scarring. We here provide a simple procedure to splint the edges of full-thickness rodent skin with a sutured plastic frame that prevents wound closure by granulation tissue contraction. The resulting mechanical tension in the wound bed and the lack of neo-epithelium amplify myofibroblast formation and generate hypertrophic features, not unlike those of human skin. In addition to producing scar tissue, the splint provides a reservoir that can be used for the delivery of cellular and acellular wound treatment regimen. Despite being simple and almost historical, wound splinting is a robust and reliable model to study myofibroblast biology.
人类的增生性瘢痕是皮肤愈合不完善的结果，尤其是在严重烧伤后形成的瘢痕中更为明显。相比之下，小鼠和大鼠的全层皮肤伤口正常愈合，不会形成可见的瘢痕组织，这降低了啮齿动物模型在皮肤瘢痕研究中的适用性。在这里，我们提供了一种简单的方法，即用缝合的塑料框架将全层啮齿动物皮肤的边缘夹在一起，防止肉芽组织收缩导致伤口闭合。由此产生的伤口床机械张力和缺乏新生上皮会放大肌成纤维细胞的形成，并产生类似于人类皮肤的增生性特征。除了产生瘢痕组织外，夹板还提供了一个可以用于输送细胞和非细胞伤口治疗方案的储库。尽管很简单，几乎是历史遗留问题，但伤口夹板是研究肌成纤维细胞生物学的一种强大而可靠的模型。
