Raje Komal, Tanaka Shoya, Fujita Satoshi
Department of Frontier Fiber Technology and Sciences, University of Fukui, Fukui 910-8507, Japan.
Life Science Innovation Center, University of Fukui, Fukui 910-8507, Japan.
ACS Omega. 2024 Sep 10;9(38):40010-40018. doi: 10.1021/acsomega.4c05851. eCollection 2024 Sep 24.
Hyaluronan (HA) is widely used in cosmetic and biomedical applications due to its excellent biocompatibility and potential to promote wound healing. Nanofibrous HA, mimicking the extracellular matrix (ECM), is considered promising for therapeutic and cosmetic applications. However, the electrospinning process of HA often necessitates cytotoxic solvents and chemical modifications, compromising its biocompatibility and advantageous properties. In this study, poly(ethylene oxide) (PEO) was added to an aqueous solution of natural HA to improve its spinnability, enabling HA to be electrospun into fibers. The HA was rendered water-insoluble by treatment with an acidic solution, and the amorphized PEO, achieved by heat-quenching, was removed through water washing. This method distinguishes it from previous reports of fibers blended with PEO or other water-soluble polymers. Consequently, the resulting HA gel fibers demonstrated suitability for mesenchymal stem cell adhesion due to the exposure of HA on the fiber surface. Additionally, HA fibers were successfully applied directly onto the skin using a hand-held electrospinning device, indicating the potential for point-of-care and home use applications.
透明质酸（HA）因其出色的生物相容性和促进伤口愈合的潜力而广泛应用于化妆品和生物医学领域。模仿细胞外基质（ECM）的纳米纤维状HA被认为在治疗和化妆品应用方面具有前景。然而，HA的静电纺丝过程通常需要使用细胞毒性溶剂并进行化学修饰，这会损害其生物相容性和有利特性。在本研究中，将聚环氧乙烷（PEO）添加到天然HA的水溶液中以改善其可纺性，使HA能够静电纺丝成纤维。通过用酸性溶液处理使HA变得不溶于水，并通过水洗去除经热猝灭得到的无定形PEO。该方法与先前关于与PEO或其他水溶性聚合物共混纤维的报道不同。因此，所得的HA凝胶纤维由于纤维表面HA的暴露而显示出适合间充质干细胞粘附。此外，使用手持式静电纺丝装置成功地将HA纤维直接应用于皮肤，表明其在即时护理和家庭使用应用方面的潜力。
