Luo Yi, Duan Mengting, Liu Runze, Liu Pei, Fan Wei, Fan Bing
State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
J Dent Sci. 2024 Oct;19(4):2226-2235. doi: 10.1016/j.jds.2024.02.027. Epub 2024 Mar 6.
BACKGROUND/PURPOSE: Root canal irrigants are difficult to diffuse deep into the dentinal tubules for root canal disinfection. The purpose of this study was to assess the ability of different diffusing enhancers to deliver chlorhexidine (CHX) into dentinal tubules.
The diffusing property of five diffusing enhancers (acetone, DMSO, Triton X-100, JFC-E and azone) into dentinal tubules was firstly assessed using the Rhodamine B. The ability of the diffusing enhancers to deliver CHX into dentinal tubules was then evaluated both qualitatively and quantitatively. A deep dentinal tubule infection model was further established to test the bactericidal effect of CHX delivered by different diffusing enhancers. Finally, the cytotoxicity of these diffusing enhancers was tested using the CCK-8 method.
Azone, Triton X-100 and JFC-E exhibited the largest maximum diffusing depths at all root canal parts ( < 0.05). Azone group also showed the highest percentage of diffusing depths for all root canal parts, followed by Triton X-100 ( < 0.05). The percentage of dead bacteria in dentinal tubules close to cementum layer was significantly higher in the CHX + azone group than in the other groups ( < 0.05), followed by CHX + Triton X-100 and CHX + JFC-E groups. The concentration of CHX diffusing onto the exterior surface of root was significantly higher in CHX + azone group than in the other groups ( < 0.05). All the diffusing enhancers showed relatively low cytotoxicity.
Azone showed the highest diffusing ability with low cytotoxicity and might be employed as a carrier agent for either intracanal irrigants or medications to achieve more thorough root canal disinfection.
背景/目的：根管冲洗剂难以深入扩散至牙本质小管进行根管消毒。本研究旨在评估不同扩散增强剂将氯己定（CHX）输送至牙本质小管的能力。
首先使用罗丹明B评估五种扩散增强剂（丙酮、二甲基亚砜、吐温X-100、JFC-E和氮酮）向牙本质小管的扩散特性。然后对扩散增强剂将CHX输送至牙本质小管的能力进行定性和定量评估。进一步建立深部牙本质小管感染模型，以测试不同扩散增强剂输送的CHX的杀菌效果。最后，使用CCK-8法测试这些扩散增强剂的细胞毒性。
氮酮、吐温X-100和JFC-E在所有根管部位均表现出最大的最大扩散深度（P<0.05）。氮酮组在所有根管部位的扩散深度百分比也最高，其次是吐温X-100（P<0.05）。靠近牙骨质层的牙本质小管中死菌百分比在CHX+氮酮组显著高于其他组（P<0.05），其次是CHX+吐温X-100组和CHX+JFC-E组。CHX+氮酮组扩散到牙根外表面的CHX浓度显著高于其他组（P<0.05）。所有扩散增强剂均表现出相对较低的细胞毒性。
氮酮具有最高的扩散能力且细胞毒性低，可作为管内冲洗剂或药物的载体，以实现更彻底的根管消毒。
