Yang Qian, Zhong Qi-Ming, Song Mei-Qing, Tong Li-Guo, Bai Chong-Zhi
Central Laboratory, Shanxi Province Hospital of Traditional Chinese Medicine, No. 70, Nanshifang Street, Taiyuan City, Shanxi Province, 030012, China.
Heliyon. 2024 Sep 17;10(18):e38034. doi: 10.1016/j.heliyon.2024.e38034. eCollection 2024 Sep 30.
Cardiomyocyte loss and myocardial fibrosis are major determinants of myocardial infarction (MI) pathological changes. Mesenchymal stem cell (MSC)-derived exosomes (exos) and Danshen decoction (DSY) have been demonstrated to mediate cardiac repair following MI. BM-MSCs exos or BM-MSCsDSY exos were intramuscularly injected into post-MI rats. On the 7th, 14th and 28th days, serum CK, LDH, α-HBDH, ALT, and AST were measured and electrocardiogram changes were monitored to identify cardiac function; Triphenyltetrazolium chloride staining, Hematein&Eosin staining, Masson trichrome staining and Transmission Electron Microscope were adopted to analyze infarct area, cardiac morphology, histopathology, and fibrosis and cardiomyocyte ultrastructure; TUNEL assay, real-time PCR and western blot were performed to detect cardiomyocyte apoptosis and autophagy. As a result, BMMSCsDSY exos are superior to BM-MSCs-exos in improvement of cardiac function, morphology, histopathology and cardiomyocyte ultrastructure, as well as in reduction of infarction area and cardiac fibrosis by inhibiting apoptosis and promoting autophagy of cardiomyocytes.
心肌细胞丢失和心肌纤维化是心肌梗死（MI）病理变化的主要决定因素。间充质干细胞（MSC）来源的外泌体（exos）和丹参汤（DSY）已被证明可介导心肌梗死后的心脏修复。将骨髓间充质干细胞外泌体或骨髓间充质干细胞丹参汤外泌体肌肉注射到心肌梗死后的大鼠体内。在第7天、14天和28天，测量血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、α-羟丁酸脱氢酶（α-HBDH）、谷丙转氨酶（ALT）和谷草转氨酶（AST），并监测心电图变化以确定心脏功能；采用氯化三苯基四氮唑染色、苏木精-伊红染色、Masson三色染色和透射电子显微镜分析梗死面积、心脏形态、组织病理学、纤维化和心肌细胞超微结构；进行TUNEL检测、实时聚合酶链反应和蛋白质免疫印迹法检测心肌细胞凋亡和自噬。结果显示，骨髓间充质干细胞丹参汤外泌体在改善心脏功能、形态、组织病理学和心肌细胞超微结构方面优于骨髓间充质干细胞外泌体，并且通过抑制心肌细胞凋亡和促进自噬来减少梗死面积和心脏纤维化。
