Department of Dermatology, College of Medicine, Chung-Ang University, Seoul, Republic of Korea.
Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Skin Res Technol. 2024 Oct;30(10):e70071. doi: 10.1111/srt.70071.
Cold plasma has shown efficacy in various dermatological applications by reduces inflammatory responses and modulating cytokine expression. Therefore, this study aimed to investigate the therapeutic effects of cold plasma on psoriasis.
In psoriasis HaCaT cells with cold plasma, we confirmed the expression of inflammatory cytokines involved in psoriasis formation and MAPK pathway, cell cycle, and apoptosis-related factors. In psoriasis-like BALB/c mice model, the effects of cold plasma treatment on skin were visually assessed. The expression of psoriasis-related factors was confirmed through qPCR, Western blotting, and Immunohistochemistry.
Cold plasma led to a reduction in inflammatory cytokines including IL-17A, IL-23A, IL-24, IL-1β, and TNF-α in the psoriasis cell line. It also modulated factors involved in the MAPK pathway and the cell cycle. In the psoriasis-like mice model, cold plasma resulted in improvements in skin thickness, erythema, scaling, and PASI. Additionally, decreases in inflammatory cytokines like INF-γ, IL-23, and S100a7 were observed, along with improvements in MAPK pathway activation, apoptosis, and other psoriasis-related factors.
Through in vitro and in vivo studies, our research highlights the potential of cold plasma as a novel therapeutic approach for psoriasis. Furthermore, cold plasma could serve as an adjunctive treatment for skin immunological diseases.
冷等离子体通过减少炎症反应和调节细胞因子表达，在各种皮肤科应用中显示出疗效。因此，本研究旨在探讨冷等离子体治疗银屑病的疗效。
在银屑病 HaCaT 细胞中应用冷等离子体，我们证实了参与银屑病形成的炎症细胞因子以及 MAPK 通路、细胞周期和细胞凋亡相关因子的表达。在银屑病样 BALB/c 小鼠模型中，通过肉眼评估冷等离子体处理对皮肤的影响。通过 qPCR、Western blot 和免疫组化来证实与银屑病相关的因子的表达。
冷等离子体导致银屑病细胞系中包括 IL-17A、IL-23A、IL-24、IL-1β 和 TNF-α 在内的炎症细胞因子减少。它还调节了 MAPK 通路和细胞周期相关的因子。在银屑病样小鼠模型中，冷等离子体导致皮肤厚度、红斑、鳞屑和 PASI 的改善。此外，观察到炎症细胞因子如 INF-γ、IL-23 和 S100a7 的减少，同时 MAPK 通路的激活、细胞凋亡和其他与银屑病相关的因子也得到改善。
通过体外和体内研究，我们的研究强调了冷等离子体作为一种治疗银屑病的新方法的潜力。此外，冷等离子体可作为皮肤免疫性疾病的辅助治疗方法。
