Department of Hepatobiliary Surgery, Qinghai Provincial Traffic Hospital, Xining, 810001, Qinghai, China.
Department of Evidence-Based Medicine and Social Medicine, School of Public Health , Chengdu Medical College, Chengdu, 610500, Sichuan, China.
Metabolomics. 2024 Aug 14;20(5):99. doi: 10.1007/s11306-024-02162-7.
The incidence of gallstones is high in Qinghai Province. However, the molecular mechanisms underlying the development of gallstones remain unclear.
In this study, we collected urine samples from 30 patients with gallstones and 30 healthy controls. The urine samples were analysed using multi-omics platforms. Proteomics analysis was conducted using data-independent acquisition, whereas metabolomics analysis was performed using liquid chromatography-mass spectrometry (LC-MS).
Among the patients with gallstones, we identified 49 down-regulated and 185 up-regulated differentially expressed proteins as well as 195 up-regulated and 189 down-regulated differentially expressed metabolites. Six pathways were significantly enriched: glycosaminoglycan degradation, arginine and proline metabolism, histidine metabolism, pantothenate and coenzyme A biosynthesis, drug metabolism-other enzymes, and the pentose phosphate pathway. Notably, 10 differentially expressed proteins and metabolites showed excellent predictive performance and were selected as potential biomarkers.
The findings of our metabolomics and proteomics analyses provide new insights into novel biomarkers for patients with cholelithiasis in high-altitude areas.
青海省胆结石的发病率很高。然而，胆结石发展的分子机制尚不清楚。
本研究收集了 30 例胆结石患者和 30 例健康对照者的尿液样本。使用多组学平台对尿液样本进行分析。蛋白质组学分析采用数据非依赖性采集方法，代谢组学分析采用液相色谱-质谱联用技术（LC-MS）。
在胆结石患者中，我们鉴定出 49 个下调和 185 个上调的差异表达蛋白，以及 195 个上调和 189 个下调的差异表达代谢物。有 6 条途径显著富集：糖胺聚糖降解、精氨酸和脯氨酸代谢、组氨酸代谢、泛酸和辅酶 A 生物合成、药物代谢-其他酶和戊糖磷酸途径。值得注意的是，10 个差异表达的蛋白和代谢物具有出色的预测性能，被选为潜在的生物标志物。
代谢组学和蛋白质组学分析的结果为高海拔地区胆石症患者提供了新的潜在生物标志物。
