College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.
Faculty of Light Industry, State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Paper Science and Technology of Ministry of Education, Qi Lu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China.
Photochem Photobiol Sci. 2024 Oct;23(10):1883-1891. doi: 10.1007/s43630-024-00637-z. Epub 2024 Oct 1.
The disruption of lipid droplet function is associated with the pathogenesis of various diseases. Clarifying the response behavior of lipid droplets to the microenvironment at the cellular level is of great significance. Plant lipids not only exist in phospholipids in cell membranes, but also in aromatic essential oils. Monitoring the level of lipid droplets in plant cells using fluorescent probes provides a simple method for screening lipid-rich varieties. We synthesized a polarity-viscosity responsive coumarin fluorescent probe, Cou-CN, which achieved sensitive detection of polarity and viscosity in dilute solution environments by constructing this simple probe with ICT and TICT properties and verifying it using Gaussian computational simulation. Cou-CN exhibited good lipid droplet illumination effects in HepG2 cells with a correlation coefficient of 0.92 compared to the commercial lipid droplet dye BODIPY. Additionally, co-staining the probe with the lipophilic commercial dye Nile Red in tobacco root stem seedling cells resulted in a high correlation coefficient of 0.9.
脂滴功能的紊乱与各种疾病的发病机制有关。阐明脂滴对细胞水平微环境的响应行为具有重要意义。植物脂质不仅存在于细胞膜的磷脂中，还存在于芳香性精油中。利用荧光探针监测植物细胞中脂滴的水平为筛选富含脂质的品种提供了一种简单的方法。我们合成了一种对极性和粘度响应的香豆素荧光探针 Cou-CN，通过构建具有 ICT 和 TICT 性质的简单探针，并通过高斯计算模拟进行验证，该探针可以在稀溶液环境中实现对极性和粘度的灵敏检测。Cou-CN 在 HepG2 细胞中具有良好的脂滴照明效果，与商业脂滴染料 BODIPY 的相关系数为 0.92。此外，将探针与亲脂性商业染料尼罗红在烟草根茎苗细胞中共同染色，得到的相关系数高达 0.9。
