State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp & Paper Science and Technology of Shandong Province, Jinan 250353, PR China; Ministry of Education, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp & Paper Science and Technology of Shandong Province, Jinan 250353, PR China; Ministry of Education, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jun 5;314:124180. doi: 10.1016/j.saa.2024.124180. Epub 2024 Mar 22.
NH is a common raw material used in the production of pesticides and has good water solubility, so it may contaminate water sources and eventually enter living organisms, causing serious health problems. Viscosity is an important indicator of the cellular microenvironment and an early warning signal for many diseases. The high reactivity of hydrazine depletes glutathione (GSH) in hepatocytes, causing oxidative stress ultimately leading to significant changes in intracellular viscosity and even death. Therefore, it is particularly important to develop an effective method to detect NH and viscosity in environmental and biological systems. On this basis, we developed two fluorescent probes, BDD and BHD, based on xanthene and 2-benzothiazole acetonitrile. The experimental results show that BHD and BDD have good imaging capabilities for NH in cells, zebrafish and Arabidopsis. BHD and BDD also showed sensitive detection and fluorescence enhancement in the near-infrared region when the intracellular viscosity was changed. Notably, the probe BDD has also successfully imaged NH in a variety of real water samples.
NH 是生产农药的常用原料，具有良好的水溶性，因此可能会污染水源，并最终进入生物体，导致严重的健康问题。黏度是细胞微环境的一个重要指标，也是许多疾病的早期预警信号。肼的高反应性会使肝细胞中的谷胱甘肽（GSH）耗竭，导致氧化应激，最终导致细胞内黏度发生显著变化，甚至死亡。因此，开发一种有效检测环境和生物系统中 NH 和黏度的方法尤为重要。在此基础上，我们基于香豆素和 2-苯并噻唑乙腈设计合成了两种荧光探针 BDD 和 BHD。实验结果表明，BHD 和 BDD 对细胞、斑马鱼和拟南芥中的 NH 具有良好的成像能力。当细胞内黏度发生变化时，BHD 和 BDD 还在近红外区域表现出了灵敏的检测和荧光增强。值得注意的是，探针 BDD 还成功地对各种实际水样中的 NH 进行了成像。
