Gershon H, Grefig A T, Cady D J
Can J Microbiol. 1985 Aug;31(8):707-10. doi: 10.1139/m85-134.
The copper(II) contents of the growth media, Sabouraud dextrose and Czapek-Dox broths, and of the spore inocula of Aspergillus niger (ATCC 1004), Aspergillus oryzae (ATCC 1011), Trichoderma viride (ATCC 8678), and Myrothecium verrucaria (ATCC 9095) were determined by atomic absorption spectrophotometry in a graphite furnace. The test systems composed of Sabouraud dextrose broth and spore inocula of the four fungi contained only a little over 3% of the copper(II) required to form a minimal inhibitory concentration of bis(8-quinolinolato)copper(II). The test system of Czapek-Dox broth and A. oryzae contained slightly less than 65% of the copper(II) required to form a minimal inhibitory concentration of the bischelate of 8-quinolinol with copper(II). When the minimal inhibitory concentrations of 8-quinolinol and bis(8-quinolinolato)copper(II) were added simultaneously to the test system of Czapek-Dox broth and A. oryzae, 10% of the combined mixture of toxicants caused complete inhibition of growth indicating synergism between the toxicants. These results together with the observation that alpha-lipoic acid as well as small aliphatic thiol-containing compounds (cysteine, glutathione, dithioerythritol, and dithiothreitol) reversed the toxicity of 8-quinolinol but not the toxicity of bis(8-quinolinolato)copper(II) led to the conclusion that the mechanisms of fungitoxicity of both toxicants are different.
采用石墨炉原子吸收分光光度法测定了生长培养基（沙氏葡萄糖肉汤和察氏肉汤）以及黑曲霉（ATCC 1004）、米曲霉（ATCC 1011）、绿色木霉（ATCC 8678）和疣孢漆斑菌（ATCC 9095）孢子接种物中的铜（II）含量。由沙氏葡萄糖肉汤和这四种真菌的孢子接种物组成的测试系统中所含的铜（II）仅略高于形成双（8 - 喹啉醇）铜（II）最低抑菌浓度所需铜（II）的3%。察氏肉汤和米曲霉的测试系统中所含的铜（II）略低于形成8 - 喹啉醇与铜（II）双螯合物最低抑菌浓度所需铜（II）的65%。当将8 - 喹啉醇和双（8 - 喹啉醇）铜（II）的最低抑菌浓度同时添加到察氏肉汤和米曲霉的测试系统中时，10%的混合毒物组合导致生长完全抑制，表明毒物之间存在协同作用。这些结果，连同α - 硫辛酸以及含脂肪族硫醇的小分子化合物（半胱氨酸、谷胱甘肽、二硫赤藓糖醇和二硫苏糖醇）能逆转8 - 喹啉醇的毒性但不能逆转双（8 - 喹啉醇）铜（II）毒性的观察结果，得出这两种毒物的杀真菌毒性机制不同的结论。
