Okba Mona M, Abdel Baki Passent M, Abu-Elghait Mohammed, Shehabeldine Amr M, El-Sherei Moshera M, Khaleel Amal E, Salem Mohamed A
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Nasr City, Cairo, Egypt.
J Ethnopharmacol. 2022 Jan 10;282:114658. doi: 10.1016/j.jep.2021.114658. Epub 2021 Sep 20.
The use of plant extracts and their phytochemicals as candidates for targeting the microbial resistance inhibition is increasingly focused in last decades. In Mongolian traditional medicine, Irises were long used for the treatment of bacterial infections. Irises have been used since the Ancient Egyptians.
Chemical composition and virulence inhibition potential of both polar (PF) and non-polar fractions (NPF) of three common Iris species (I. confusa, I. pseudacorus and I. germanica) were explored.
Secondary metabolites profiling was characterized by the UPLC-HRMS/MS technique. Multi-variate data analysis was performed using Metaboanalyst 3.0. Anti-virulence inhibitory activity was evaluated via anti-haemolytic assay and Quantitative biofilm inhibition assay.
I. pseudacorus PF exhibited the most potent effect against S. aureus haemolytic activity. All the tested fractions from all species, except I. pseudacorus NPF, have no significant inhibition on the biofilm formation of methicillin resistant and sensitive (MRSA and MSSA) S. aureus. I. pseudacorus NPF showed potent biofilm inhibitory potential of 71.4 and 85.8% against biofilm formation of MRSA and MSSA, respectively. Metabolite profiling of the investigated species revealed ninety and forty-five metabolites detected in the PFs and NPFs, respectively. Nigricin-type, tectorigenin-type isoflavonids and xanthones allowed the discrimination of I. pseudacorus PF from the other species, highlighting the importance of those metabolites in exerting its promising activity. On the other hand, triterpene acids, iridals, triacylglycerols and ceramides represented the metabolites detected in highest abundance in I. pseudacorus NPF.
This is the sole map represents the secondary metabolites profiling of the PFs and NPFs of common Iris species correlating them with the potent explored Staphylococcus aureus anti-virulence activity.
在过去几十年中，植物提取物及其植物化学物质作为针对微生物耐药性抑制的候选物的应用越来越受到关注。在蒙古传统医学中，鸢尾长期以来被用于治疗细菌感染。鸢尾自古埃及就已被使用。
探究三种常见鸢尾物种（混淆鸢尾、黄菖蒲和德国鸢尾）的极性部分（PF）和非极性部分（NPF）的化学成分及毒力抑制潜力。
采用超高效液相色谱-高分辨质谱/质谱技术对次生代谢产物进行分析。使用Metaboanalyst 3.0进行多变量数据分析。通过抗溶血试验和定量生物膜抑制试验评估抗毒力抑制活性。
黄菖蒲PF对金黄色葡萄球菌溶血活性表现出最显著的作用。除黄菖蒲NPF外，所有物种的所有测试部分对耐甲氧西林和敏感金黄色葡萄球菌（MRSA和MSSA）的生物膜形成均无显著抑制作用。黄菖蒲NPF对MRSA和MSSA的生物膜形成分别显示出71.4%和85.8%的强效生物膜抑制潜力。对所研究物种的代谢产物分析表明，在PF和NPF中分别检测到90种和45种代谢产物。黑麦角硫因型、鸢尾黄酮型异黄酮和氧杂蒽酮可将黄菖蒲PF与其他物种区分开来，突出了这些代谢产物在发挥其有前景的活性方面的重要性。另一方面，三萜酸、鸢尾酮、三酰甘油和神经酰胺是在黄菖蒲NPF中检测到的含量最高的代谢产物。
这是唯一一张展示常见鸢尾物种PF和NPF次生代谢产物图谱，并将它们与所探究的金黄色葡萄球菌抗毒力活性相关联的图谱。
