Prasad Tanya, Pawar Renuka, Ganiger Chanamallappa, Ronad Yusuf, Phaphe Sandesh, Mane Pratap, Patil Seema
Orthodontics and Dentofacial Orthopedics, School of Dental Sciences, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, IND.
Cureus. 2024 Aug 31;16(8):e68346. doi: 10.7759/cureus.68346. eCollection 2024 Aug.
Introduction The goal of orthodontic treatment is to provide patients with esthetic smiles and functional occlusion. Despite best efforts and continuous evolution of materials, white spot lesions present a persistent hindrance to the desired treatment outcome. Nanoparticles have shown efficacy in reducing microbial activity; however, currently, there is a need for natural anti-cariogenic compounds with minimal side effects. Resveratrol is a natural compound belonging to the polyphenol group and has shown promising anti-microbial efficacy. This study aimed to evaluate the influence of dentin bonding agents incorporated with the following three different nanoparticles on shear bond strength: silver nanoparticles (Ag-Np), zinc oxide nanoparticles (ZnO-Np), and resveratrol nanoparticles (RSV-Np). Materials and methods A total of 40 premolar teeth therapeutically extracted were assigned to four equal groups of n=10 each. Groups 1, 2, and 3 used experimental adhesives doped with silver, zinc oxide, and resveratrol nanoparticles, respectively. Group 4 was bonded using unmodified adhesive. The bonded teeth were then subjected to shear bond strength (SBS) testing which was measured using a Universal Testing Machine (model no. UNITEST-10; Pune, India: ACME Engineers). Statistical analyses were performed using SPSS version 21 (Armonk, NY: IBM Corp.), employing one-way ANOVA and Tukey's post-hoc test for pairwise comparisons. Results Shear bond strength testing revealed that the control group with unmodified adhesive (8.6 MPa) had the highest SBS, followed by RSV-Np (7.6 MPa), Ag-Np (6.3 MPa), and ZnO-Np (5.65 MPa). Although the experimental groups demonstrated decreased SBS compared to the control, the values for Ag-Np and RSV-Np fell within the acceptable range. Conclusion Resveratrol nanoparticles had the least impact on shear bond strength among the experimental groups. These findings suggest that the incorporation of resveratrol nanoparticles in dentin bonding agents can provide anti-cariogenic effect without significantly impacting the adhesive's mechanical properties thereby providing a new and promising alternative to synthetic nanoparticles. Further studies are recommended to optimize the balance between anti-microbial efficacy and bond strength in clinical applications.
引言 正畸治疗的目标是为患者提供美观的笑容和功能性咬合。尽管材料不断发展且正畸医生已竭尽全力，但白斑病变仍然是实现理想治疗效果的持续障碍。纳米颗粒已显示出降低微生物活性的功效；然而，目前需要副作用最小的天然抗龋化合物。白藜芦醇是一种属于多酚类的天然化合物，已显示出有前景的抗微生物功效。本研究旨在评估掺入以下三种不同纳米颗粒的牙本质粘结剂对剪切粘结强度的影响：银纳米颗粒（Ag-Np）、氧化锌纳米颗粒（ZnO-Np）和白藜芦醇纳米颗粒（RSV-Np）。
材料与方法 总共40颗治疗性拔除的前磨牙被分为四组，每组n = 10。第1组、第2组和第3组分别使用掺杂银、氧化锌和白藜芦醇纳米颗粒的实验性粘结剂。第4组使用未改性的粘结剂进行粘结。然后对粘结的牙齿进行剪切粘结强度（SBS）测试，使用万能试验机（型号：UNITEST - 10；印度浦那：ACME工程师公司）进行测量。使用SPSS 21版（纽约州阿蒙克：IBM公司）进行统计分析，采用单因素方差分析和Tukey事后检验进行两两比较。
结果 剪切粘结强度测试显示，使用未改性粘结剂的对照组（8.6 MPa）具有最高的SBS，其次是RSV-Np（7.6 MPa）、Ag-Np（6.3 MPa）和ZnO-Np（5.65 MPa）。尽管实验组的SBS与对照组相比有所降低，但Ag-Np和RSV-Np的值仍在可接受范围内。
结论 在实验组中，白藜芦醇纳米颗粒对剪切粘结强度的影响最小。这些发现表明，在牙本质粘结剂中掺入白藜芦醇纳米颗粒可以提供抗龋效果，而不会显著影响粘结剂的机械性能，从而为合成纳米颗粒提供了一种新的、有前景的替代方案。建议进一步研究以优化临床应用中抗微生物功效和粘结强度之间的平衡。
