Almeshal Reem, Pagni Sarah, Ali Ala, Zoukhri Driss
College of Dentistry, Tufts University, Boston, USA.
Department of Public Health and Community Service, Tufts University School of Dental Medicine, Boston, USA.
Cureus. 2024 Feb 12;16(2):e54098. doi: 10.7759/cureus.54098. eCollection 2024 Feb.
White spot lesions are common after orthodontic treatment. Chitosan nanoparticles (CS-NPs) have emerged as promising antibacterial agents that inhibit the growth of . The aim of the study was to investigate the nano-effect of adhesives containing CS-NPs on and their effects on shear bond strength.
The inhibitory effects of two sizes of CS-NPs were assessed using the disc agar diffusion method. Four wells were created in the petri dishes, and each was inoculated with broth (negative control), chlorhexidine (positive control), CS-NPs (20 nm), or CS-NPs (131 nm). An Instron machine was used to evaluate shear bond strength by allocating 24 teeth into three groups, and all measurements were recorded in megapascals. Caries progression was assessed using the International Caries Detection and Assessment System and surface profilometry. Statistical analysis was performed using IBM SPSS Statistics for Windows, Version 27.0 (Released 2020; IBM Corp., Armonk, New York, United States) for a one-way ANOVA followed by Tukey's multiple comparison test.
Disc agar diffusion showed a reduction in in the CS-NP group compared to the control (p < 0.001), with no statistical significance between the sizes of 20 and 131 nm (p = 0.95). Regarding shear bond strength, no differences were recorded when adhesive-containing CS-NPs and the control were compared (p = 0.44). Additionally, no differences were found within the CS-NP groups (p = 0.91). Caries assessments showed excellent agreement, as indicated by a weighted kappa. Profilometry readings showed higher surface roughness in the control than in the CS-NP groups (p < 0.001), with no statistically significant difference between the CS-NP groups (p = 0.72).
CS-NPs of both sizes tested had similar antibacterial effects. In addition, the incorporation of CS-NPs did not affect shear bond strength.
正畸治疗后白斑病变很常见。壳聚糖纳米颗粒（CS-NPs）已成为有前景的抗菌剂，可抑制……的生长。本研究的目的是研究含CS-NPs的粘合剂的纳米效应及其对剪切粘结强度的影响。
使用圆盘琼脂扩散法评估两种尺寸的CS-NPs的抑制作用。在培养皿中创建四个孔，每个孔接种肉汤（阴性对照）、洗必泰（阳性对照）、CS-NPs（20纳米）或CS-NPs（131纳米）。使用英斯特朗机器通过将24颗牙齿分为三组来评估剪切粘结强度，所有测量值均以兆帕为单位记录。使用国际龋病检测与评估系统和表面轮廓测量法评估龋病进展。使用IBM SPSS Statistics for Windows，版本27.0（2020年发布；IBM公司，美国纽约州阿蒙克）进行统计分析，采用单因素方差分析，随后进行Tukey多重比较检验。
圆盘琼脂扩散显示，与对照组相比，CS-NP组中……减少（p < 0.001），20纳米和131纳米尺寸之间无统计学意义（p = 0.95）。关于剪切粘结强度，比较含CS-NPs的粘合剂和对照组时未记录到差异（p = 0.44）。此外，CS-NP组内也未发现差异（p = 0.91）。龋病评估显示一致性良好，加权kappa表明了这一点。轮廓测量读数显示对照组的表面粗糙度高于CS-NP组（p < 0.001），CS-NP组之间无统计学显著差异（p = 0.72）。
测试的两种尺寸的CS-NPs具有相似的抗菌效果。此外，CS-NPs的加入不影响剪切粘结强度。
