Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Shandong 276005, China.
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Life Science, Linyi University, Shandong 276005, China.
Langmuir. 2024 Oct 15;40(41):21327-21340. doi: 10.1021/acs.langmuir.4c02804. Epub 2024 Oct 2.
Cancer, which is characterized by high mortality rates, poses a significant threat to global human health. Early diagnosis is of paramount importance in managing cancer, and tumor markers have emerged as crucial indicators for achieving this goal. The advent of precision medicine has further emphasized the need for the effective detection of these markers. However, traditional detection methods are hampered by numerous limitations. In recent years, nanopore technology has emerged as a promising alternative, due to its unique physical and chemical properties, which facilitate rapid, label-free, and amplification-free detection. This Review focuses on the direct detection of tumor markers through nucleic acid analysis and indirect detection mediated by nucleic acids and facilitated by biological nanopores. Furthermore, it also discusses the challenges and prospects of applying biological nanopore sensing technology in early cancer diagnosis, underscoring its potential to revolutionize tumor marker detection.
癌症死亡率高，对全球人类健康构成重大威胁。癌症的早期诊断对于癌症的管理至关重要，肿瘤标志物已成为实现这一目标的关键指标。精准医学的出现进一步强调了有效检测这些标志物的必要性。然而，传统的检测方法受到许多限制。近年来，由于其独特的物理和化学性质，纳米孔技术作为一种很有前途的替代方法出现了，这使得它能够实现快速、无标记和无需扩增的检测。这篇综述主要关注通过核酸分析进行的肿瘤标志物的直接检测以及通过核酸介导并借助生物纳米孔进行的间接检测。此外，还讨论了将生物纳米孔传感技术应用于早期癌症诊断中所面临的挑战和前景，强调了它在肿瘤标志物检测方面具有革命性的潜力。
