College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Northeast Forestry University, Harbin 150040, PR China.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Northeast Forestry University, Harbin 150040, PR China.
Int J Biol Macromol. 2024 Nov;280(Pt 4):136168. doi: 10.1016/j.ijbiomac.2024.136168. Epub 2024 Sep 30.
Sporopollenin is a natural biological macromolecule consisting of highly cross-linked carbon, hydrogen, and oxygen atoms, with a highly porous structure and multifunctional groups. In this work, a novel surface molecularly imprinted polymer based on magnetically aminated cattail sporopollenin (MACSp-SMIP) was prepared for the specific and efficient adsorption of resveratrol, with the aim of purifying resveratrol from Polygonum cuspidatum extracts. MACSp-SMIP was found to have a porous structure covered with the multi-layered sponge-like imprinted polymers. MACSp-SMIP had a high adsorption capacity for resveratrol (65.77 mg·g) and excellent selectivity (imprinting factor 5.64). The adsorption of resveratrol by MACSp-SMIP was a homogeneous diffusion dominated by chemical adsorption with three stages of external diffusion, internal diffusion, and micropore diffusion. MACSp-SMIP was used as an adsorbent in molecularly imprinted solid-phase extraction for the purification of resveratrol from P. cuspidatum extracts, achieving a resveratrol recovery of 94.33 % and a purity of 76.67 % in the final products. MACSp-SMIP maintained a satisfactory recovery of resveratrol (88.18 %) after six cycles. Overall, this work developed a promising biological macromolecule-based adsorbent MACSp-SMIP for the specific and efficient adsorption of resveratrol, and also provided an efficient and simple approach for the selective purification of resveratrol from P. cuspidatum extracts for food/nutraceutical applications.
类脂孢粉素是一种由高度交联的碳、氢和氧原子组成的天然生物大分子，具有高度多孔的结构和多功能基团。在这项工作中，制备了一种基于磁性氨化香蒲类脂孢粉素（MACSp-SMIP）的新型表面分子印迹聚合物，用于白藜芦醇的特异性和高效吸附，旨在从虎杖提取物中纯化白藜芦醇。MACSp-SMIP 被发现具有多孔结构，表面覆盖着多层海绵状印迹聚合物。MACSp-SMIP 对白藜芦醇具有高吸附容量（65.77 mg·g）和优异的选择性（印迹因子 5.64）。MACSp-SMIP 对白藜芦醇的吸附是一个由化学吸附主导的均相扩散过程，分为三个阶段：外部扩散、内部扩散和微孔扩散。MACSp-SMIP 被用作分子印迹固相萃取中的吸附剂，用于从虎杖提取物中纯化白藜芦醇，最终产物中白藜芦醇的回收率为 94.33%，纯度为 76.67%。MACSp-SMIP 在经过六次循环后仍能保持令人满意的白藜芦醇回收率（88.18%）。总的来说，这项工作开发了一种有前途的基于生物大分子的吸附剂 MACSp-SMIP，用于白藜芦醇的特异性和高效吸附，还为食品/营养保健品应用中从虎杖提取物中选择性纯化白藜芦醇提供了一种高效、简单的方法。
