Department of Pharmacodynamics and Toxicology, Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
J Sep Sci. 2010 Aug;33(15):2302-9. doi: 10.1002/jssc.201000183.
A new molecularly imprinted polymer for extraction of crocin from saffron stigmas was prepared using gentiobiose (a glycoside moiety in crocin structure) as a template. Crocin binding to gentiobiose imprinted polymer (Gent-MIP) was studied in comparison with a blank nonimprinted polymer in aqueous media. Affinity of the Gent-MIP for the crocin was more than the nonimprinted polymer at all concentrations. In Scatchard analysis, the number of binding sites in each gram of polymer (maximum binding sites) and dissociation constant of crocin to binding sites were 18.4 micromol/g polymer and 11.2 microM, respectively. The Gent-MIP was then used as the sorbent in an SPE method for isolation and purification of crocin from methanolic extract of saffron stigmas. The recovery of crocin, safranal and picrocrocin was determined in washing and elution steps. The Gent-MIP had significantly higher affinity for crocin than other compounds and enabled selective extraction of crocin with a high recovery (84%) from a complex mixture. The results demonstrated the possibility of using a part of a big molecule in preparing a molecularly imprinted polymer with a good selectivity for the main structure.
一种新的分子印迹聚合物，用于从藏红花柱头中提取藏红花素，使用龙胆二糖（藏红花素结构中的糖苷部分）作为模板进行制备。在水介质中，将藏红花素与龙胆二糖印迹聚合物（Gent-MIP）的结合与空白非印迹聚合物进行了比较研究。在所有浓度下，Gent-MIP 对藏红花素的亲和力均高于非印迹聚合物。在 Scatchard 分析中，每克聚合物中的结合位点数量（最大结合位点）和藏红花素与结合位点的解离常数分别为 18.4 微摩尔/克聚合物和 11.2 微摩尔。然后，Gent-MIP 被用作 SPE 方法中的吸附剂，用于从藏红花柱头的甲醇提取物中分离和纯化藏红花素。在洗涤和洗脱步骤中确定了藏红花素、藏红花醛和苦藏花素的回收率。Gent-MIP 对藏红花素的亲和力明显高于其他化合物，能够从复杂混合物中选择性地提取藏红花素，回收率高达 84%。结果表明，使用大分子的一部分制备对主要结构具有良好选择性的分子印迹聚合物是可行的。
