Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", 80131 Naples, Italy.
Department of Clinical Medicine and Surgery, the University of Naples "Federico II", 80131 Naples, Italy.
Dent Mater. 2024 Nov;40(11):2043-2050. doi: 10.1016/j.dental.2024.09.019. Epub 2024 Oct 2.
This study investigated the impact of some specific experimental calcium phosphate cements doped with different fluoride salts (FDCPCs) concentrations on the basal functions of human Dental Pulp Stem Cells (hDPSCs). Furthermore, this study also examined the migration, as well as the mineralisation through osteogenic differentiation.
Experimental FDCPCs were formulated using different concentrations of calcium/sodium fluoride salts [(5 wt%: VS5F), (10 wt%: VS10F), (20 wt%: VS20F)]. A fluoride-free calcium phosphate (VS0F) was used as a control. The hDPSCs were assessed to evaluate their self-renewal and migration activity in the presence of eluates of the different FDCPCs. A viability assay in osteogenic conditions was carried out, along with the differentiation potential through Alkaline Phosphatase Activity (ALP), and Alizarin Red Staining (ARS). Moreover, the gene expression of specific markers (RUNX2, ALP, COL1α1, OCN, OPN, DSPP, MEPE, and DMP-1) was also evaluated.
All the tested FDCPD had no influence on cell migrations, but they caused a decrease in cell viability in osteogenic conditions when not diluted. Conversely, the eluants of VS20F showed a positive effect on stem cell differentiation. This result was corroborated through ALP activity, ARS assay. Moreover, upregulation of specific gene markers such as RUNX2, DMP-1, and DSPP was observed in hDPSCs, especially when treated with VS20F.
The experimental FDCPC tested in this study exhibits a dose-dependent capacity to promote mineralisation in osteogenic environment. The FDCPC-VS20F seems to be the most promising experimental material suitable for developing of pulp-capping materials with osteogenic and bioactive properties.
本研究旨在探讨不同氟化物盐（FDCPCs）浓度掺杂的特定实验磷酸钙水泥对人牙髓干细胞（hDPSCs）基本功能的影响。此外，本研究还研究了迁移以及通过成骨分化的矿化。
使用不同浓度的钙/氟化钠盐（[5wt%：VS5F]，[10wt%：VS10F]，[20wt%：VS20F]）配制实验性 FDCPCs。使用无氟磷酸钙（VS0F）作为对照。评估 hDPSCs，以评估其在不同 FDCPC 洗脱液存在下的自我更新和迁移活性。在成骨条件下进行了活力测定，以及通过碱性磷酸酶活性（ALP）和茜素红染色（ARS）进行分化潜力。此外，还评估了特定标记物（RUNX2、ALP、COL1α1、OCN、OPN、DSPP、MEPE 和 DMP-1）的基因表达。
所有测试的 FDCPD 对细胞迁移均无影响，但在未稀释的情况下，在成骨条件下会降低细胞活力。相反，VS20F 的洗脱液对干细胞分化有积极影响。这一结果通过 ALP 活性和 ARS 测定得到证实。此外，在 hDPSCs 中观察到特定基因标记物（如 RUNX2、DMP-1 和 DSPP）的上调，尤其是在用 VS20F 处理时。
本研究中测试的实验性 FDCPC 具有在成骨环境中促进矿化的剂量依赖性能力。FDCPC-VS20F 似乎是最有前途的实验材料，适合开发具有成骨和生物活性的牙髓覆盖材料。
