Setiawan Eko, Putra Agung, Nabih Dimas Irfan, Ovaditya Shafira Zahra, Rizaldy Rheza
Department of Surgery, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang.
Stem Cell and Cancer Research (SCCR) Laboratory, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang.
Ann Med Surg (Lond). 2024 Aug 22;86(10):5776-5783. doi: 10.1097/MS9.0000000000002395. eCollection 2024 Oct.
Intestinal perforation has significant fatality due to sepsis contamination and prolonged inflammation. Studies showed that mesenchymal stem cells (MSCs) secreted cytokines and growth factors to reduce inflammation. This study aims to reveal the role of MSCs in controlling inflammation in intestinal perforation wound healing by measuring interleukin-6 (IL-6) and leukocytes in injured tissue.
A total of 48 rat models with a 10-mm longitudinal incision at the small intestine were divided into four groups: sham, control, Treatment group 1 (T1) injected with MSC doses of 1.5×10 cells and Treatment group 2 (T2) with 3×10 cells. IL-6 expressions were determined using western blot analysis, whereas the leukocyte infiltrations were assessed using the histopathological examination. All variables were evaluated on day 3 and 7.
Leukocyte infiltration is significantly lower in T1 and T2 compared to control group in day 3 and 7 (<0.05), while there were no differences between the two treatment groups. The expression of IL-6 was found to be significantly lower in the T1 and T2 groups compared to the control group on days 3 and 7 (<0.05), with no significant differences observed between the two treatment groups.
MSCs administration in rats with intestinal perforation reduced inflammation by controlling leukocyte infiltration and IL-6 expression.
由于脓毒症污染和炎症迁延，肠穿孔具有显著的致死率。研究表明，间充质干细胞（MSCs）分泌细胞因子和生长因子以减轻炎症。本研究旨在通过测量损伤组织中的白细胞介素-6（IL-6）和白细胞，揭示间充质干细胞在控制肠穿孔伤口愈合炎症中的作用。
总共48只在小肠处有一个10毫米纵向切口的大鼠模型被分为四组：假手术组、对照组、注射1.5×10个细胞剂量间充质干细胞的治疗组1（T1）和注射3×10个细胞剂量间充质干细胞的治疗组2（T2）。使用蛋白质印迹分析确定IL-6表达，而使用组织病理学检查评估白细胞浸润情况。所有变量在第3天和第7天进行评估。
在第3天和第7天，T1组和T2组的白细胞浸润明显低于对照组（<0.05），而两个治疗组之间没有差异。在第3天和第7天，发现T1组和T2组的IL-6表达明显低于对照组（<0.05），两个治疗组之间未观察到显著差异。
给肠穿孔大鼠施用间充质干细胞可通过控制白细胞浸润和IL-6表达来减轻炎症。
