促甲状腺激素释放激素在大鼠中枢神经系统中的免疫组织化学定位：与放射免疫分析研究的比较。

Immunohistochemical localization of TRH in rat CNS: comparison with RIA studies.

作者信息

Kreider M S, Engber T M, Nilaver G, Zimmerman E A, Winokur A

出版信息

Peptides. 1985 Sep-Oct;6(5):997-1000. doi: 10.1016/0196-9781(85)90334-1.

Abstract

The localization of thyrotropin releasing hormone (TRH) in rat brain determined by use of avidin-biotin immunoperoxidase histochemistry was compared with the distribution and quantitation by radioimmunoassay (RIA). Male Sprague-Dawley rats received intracisternal injections of 100 micrograms of colchicine or saline and were sacrificed 24 hours later. Brains were either perfused with lysine-periodate fixative and processed for TRH immunohistochemistry or were dissected into 9 brain regions for TRH RIA. In colchicine pretreated rats. TRH immunoreactive perikarya were observed only in nuclei of the hypothalamus and brain stem. No cell body staining was observable in non-colchicine treated rats. With the exception of the olfactory bulb, brain regions exhibiting dense TRH staining contained high concentrations of TRH as measured by RIA. Colchicine pretreatment did not alter the concentration of TRH in most brain regions, however, there was a significant increase in brain stem TRH content 24 hours following colchicine administration. These findings indicate that immunohistochemical localization of TRH corresponds well with endogenous concentrations of TRH determined by RIA.

摘要

采用抗生物素蛋白-生物素免疫过氧化物酶组织化学方法确定促甲状腺激素释放激素（TRH）在大鼠脑内的定位，并与放射免疫测定法（RIA）的分布和定量结果进行比较。雄性Sprague-Dawley大鼠经脑池内注射100微克秋水仙碱或生理盐水，24小时后处死。将大鼠脑用高碘酸赖氨酸固定剂灌注并进行TRH免疫组织化学处理，或将其解剖为9个脑区进行TRH RIA测定。在秋水仙碱预处理的大鼠中，仅在下丘脑和脑干的核内观察到TRH免疫反应性核周体。在未用秋水仙碱处理的大鼠中未观察到细胞体染色。除嗅球外，通过RIA测定显示，呈现密集TRH染色的脑区含有高浓度的TRH。秋水仙碱预处理并未改变大多数脑区的TRH浓度，然而，在给予秋水仙碱24小时后，脑干TRH含量显著增加。这些结果表明，TRH的免疫组织化学定位与通过RIA测定的TRH内源性浓度非常吻合。