Erin N, Okar I, Oktay S, Ercan F, Arbak S, Yeğen B C
Department of Histology, Marmara University School of Medicine, Istanbul, Turkey.
Dig Dis Sci. 1996 Jan;41(1):55-64. doi: 10.1007/BF02208584.
In the present study, two ulcer models--central thyrotropin-releasing hormone (TRH) injection and cold-restraint stress (CRS) application--were compared. Animals were treated either with salmon calcitonin (sCT) or saline intracerebroventricularly (ICV) before CRS exposure or ICV TRH injection. In both models, besides ultrastructural properties, ulcer indexes and lipid peroxidation (LP) and glutathione (GSH) levels of liver and stomach were determined. While TRH treatment did not affect GSH and LP levels of the stomach and led to a slight decrease in hepatic GSH levels, CRS induced a marked reduction in gastric and hepatic GSH and an increase in LP levels of both tissues. sCT pretreatment prevented the reduction of gastric and hepatic GSH levels and morphological damage of both tissues in the CRS group. However, the same treatment did not prevent the TRH-induced reduction of hepatic GSH levels and, interestingly, it worsened the ultrastructural disturbances in the liver. Although sCT prevented macroscopic ulcer formation in both models, it did not totally reverse the microscopic effects of TRH.
在本研究中，对两种溃疡模型——中枢促甲状腺激素释放激素（TRH）注射和冷束缚应激（CRS）施加——进行了比较。在暴露于CRS或注射脑室注射TRH之前，动物分别接受鲑鱼降钙素（sCT）或生理盐水脑室注射（ICV）治疗。在两种模型中，除了超微结构特性外，还测定了溃疡指数以及肝脏和胃的脂质过氧化（LP）和谷胱甘肽（GSH）水平。TRH治疗不影响胃的GSH和LP水平，并导致肝脏GSH水平略有下降，而CRS导致胃和肝脏GSH显著降低以及两个组织的LP水平升高。sCT预处理可防止CRS组胃和肝脏GSH水平降低以及两个组织的形态学损伤。然而，相同的治疗并不能防止TRH诱导的肝脏GSH水平降低，有趣的是，它加剧了肝脏的超微结构紊乱。尽管sCT在两种模型中均能防止宏观溃疡形成，但它并未完全逆转TRH的微观效应。
