• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种独特的细胞分裂蛋白，对细菌分裂体的组装至关重要。

A unique cell division protein critical for the assembly of the bacterial divisome.

机构信息

Department of Respiratory Medicine, Infectious Diseases and Pathogen Biology Center State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, The First Hospital of Jilin University, Changchun, China.

Bioinformatics Laboratory, The First Hospital of Jilin University, Changchun, China.

出版信息

Elife. 2024 Oct 3;12:RP87922. doi: 10.7554/eLife.87922.

DOI:10.7554/eLife.87922
PMID:39361022
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11449484/
Abstract

Identification of unique essential bacterial genes is important for not only the understanding of their cell biology but also the development of new antimicrobials. Here, we report a previously unrecognized core component of the divisome. Our results reveal that the protein, termed Aeg1 interacts with multiple cell division proteins, including FtsN, which is required for components of the divisome to localize to the midcell. We demonstrate that the FtsA and FtsB mutants effectively bypassed the need of Aeg1 by , as did the activation variants FtsW and FtsW. Our results suggest that Aeg1 is a cell division protein that arrives at the division site to initiate cell division by recruiting FtsN, which activates FtsQLB and FtsA to induce the septal peptidoglycan synthase FtsWI. The discovery of the new essential cell division protein has provided a new target for the development of antibacterial agents.

摘要

鉴定独特的必需细菌基因不仅对了解其细胞生物学很重要，而且对开发新的抗菌药物也很重要。在这里，我们报告了一个以前未被识别的分裂体核心成分。我们的结果表明，这种被称为 Aeg1 的蛋白质与多种细胞分裂蛋白相互作用，包括 FtsN，FtsN 是将分裂体组件定位到细胞中部所必需的。我们证明，FtsA 和 FtsB 突变体有效地通过 FtsW 和 FtsW 的激活变体绕过了对 Aeg1 的需求。我们的结果表明，Aeg1 是一种细胞分裂蛋白，通过招募 FtsN 到达分裂部位，激活 FtsQLB 和 FtsA 诱导隔膜肽聚糖合酶 FtsWI，从而启动细胞分裂。新的必需细胞分裂蛋白的发现为抗菌剂的开发提供了一个新的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/915591cfe4db/elife-87922-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/338573d18381/elife-87922-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/c75c53f995c2/elife-87922-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/22e69c107e41/elife-87922-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/694e5fb1fb44/elife-87922-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/602a300dc542/elife-87922-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/83cdd824c9d2/elife-87922-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/5dc40d682c71/elife-87922-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/a9a0037826c9/elife-87922-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/3d980b82c151/elife-87922-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/4ece36213f51/elife-87922-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/5c7f4907c562/elife-87922-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/915591cfe4db/elife-87922-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/338573d18381/elife-87922-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/c75c53f995c2/elife-87922-fig1-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/22e69c107e41/elife-87922-fig1-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/694e5fb1fb44/elife-87922-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/602a300dc542/elife-87922-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/83cdd824c9d2/elife-87922-fig3-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/5dc40d682c71/elife-87922-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/a9a0037826c9/elife-87922-fig4-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/3d980b82c151/elife-87922-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/4ece36213f51/elife-87922-fig5-figsupp1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/5c7f4907c562/elife-87922-fig5-figsupp2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e28b/11449484/915591cfe4db/elife-87922-fig6.jpg

相似文献

1
A unique cell division protein critical for the assembly of the bacterial divisome.一种独特的细胞分裂蛋白，对细菌分裂体的组装至关重要。
Elife. 2024 Oct 3;12:RP87922. doi: 10.7554/eLife.87922.
2
Essential Role for FtsL in Activation of Septal Peptidoglycan Synthesis.FtsL 在隔膜肽聚糖合成激活中的基本作用。
mBio. 2020 Dec 8;11(6):e03012-20. doi: 10.1128/mBio.03012-20.
3
Cell Cycle-Dependent Recruitment of FtsN to the Divisome in Escherichia coli.细胞周期依赖性 FtsN 在大肠杆菌分裂体中的募集。
mBio. 2022 Aug 30;13(4):e0201722. doi: 10.1128/mbio.02017-22. Epub 2022 Aug 15.
4
Regulation of the Peptidoglycan Polymerase Activity of PBP1b by Antagonist Actions of the Core Divisome Proteins FtsBLQ and FtsN.通过核心分裂体蛋白 FtsBLQ 和 FtsN 的拮抗作用调节 PBP1b 的肽聚糖聚合酶活性。
mBio. 2019 Jan 8;10(1):e01912-18. doi: 10.1128/mBio.01912-18.
5
A conserved subcomplex within the bacterial cytokinetic ring activates cell wall synthesis by the FtsW-FtsI synthase.细菌细胞分裂环内的一个保守亚基复合物通过 FtsW-FtsI 合成酶激活细胞壁合成。
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23879-23885. doi: 10.1073/pnas.2004598117. Epub 2020 Sep 9.
6
FtsEX acts on FtsA to regulate divisome assembly and activity.FtsEX作用于FtsA以调节分裂体的组装和活性。
Proc Natl Acad Sci U S A. 2016 Aug 23;113(34):E5052-61. doi: 10.1073/pnas.1606656113. Epub 2016 Aug 8.
7
Disruption of divisome assembly rescued by FtsN-FtsA interaction in .在. 中，通过 FtsN-FtsA 相互作用挽救了分裂体组装的破坏。
Proc Natl Acad Sci U S A. 2018 Jul 17;115(29):E6855-E6862. doi: 10.1073/pnas.1806450115. Epub 2018 Jul 2.
8
The bypass of ZipA by overexpression of FtsN requires a previously unknown conserved FtsN motif essential for FtsA-FtsN interaction supporting a model in which FtsA monomers recruit late cell division proteins to the Z ring.通过FtsN过表达绕过ZipA需要一个以前未知的保守FtsN基序，该基序对于FtsA-FtsN相互作用至关重要，这支持了一种模型，即FtsA单体将晚期细胞分裂蛋白招募到Z环。
Mol Microbiol. 2015 Mar;95(6):971-87. doi: 10.1111/mmi.12907. Epub 2015 Feb 4.
9
PBP1A Directly Interacts with the Divisome Complex to Promote Septal Peptidoglycan Synthesis in Acinetobacter baumannii.PBP1A 直接与分裂复合物相互作用，以促进鲍曼不动杆菌中隔膜肽聚糖的合成。
J Bacteriol. 2022 Dec 20;204(12):e0023922. doi: 10.1128/jb.00239-22. Epub 2022 Nov 1.
10
Structural Analysis of the Interaction between the Bacterial Cell Division Proteins FtsQ and FtsB.细菌细胞分裂蛋白 FtsQ 和 FtsB 相互作用的结构分析。
mBio. 2018 Sep 11;9(5):e01346-18. doi: 10.1128/mBio.01346-18.

本文引用的文献

1
FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli.FtsN 通过与大肠杆菌中特定于隔膜的肽聚糖合成酶形成一个连续的复合物来维持活跃的隔膜细胞壁合成。
Nat Commun. 2022 Sep 30;13(1):5751. doi: 10.1038/s41467-022-33404-8.
2
Acinetobacter baumannii.鲍曼不动杆菌
Trends Microbiol. 2022 Feb;30(2):199-200. doi: 10.1016/j.tim.2021.11.008. Epub 2021 Nov 24.
3
FtsA acts through FtsW to promote cell wall synthesis during cell division in .FtsA 通过 FtsW 在 细胞分裂过程中促进细胞壁合成。
Proc Natl Acad Sci U S A. 2021 Aug 31;118(35). doi: 10.1073/pnas.2107210118.
4
Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexes.基因分析隔肽聚糖合酶 FtsWI 复合物支持 SEDS-bPBP 复合物的保守激活机制。
PLoS Genet. 2021 Apr 15;17(4):e1009366. doi: 10.1371/journal.pgen.1009366. eCollection 2021 Apr.
5
Essential Gene Analysis in Acinetobacter baumannii by High-Density Transposon Mutagenesis and CRISPR Interference.高密度转座子诱变和 CRISPR 干扰分析鲍曼不动杆菌的必需基因。
J Bacteriol. 2021 May 20;203(12):e0056520. doi: 10.1128/JB.00565-20. Epub 2021 Mar 29.
6
A set of shuttle plasmids for gene expression in Acinetobacter baumannii.一组穿梭质粒，用于鲍曼不动杆菌中的基因表达。
PLoS One. 2021 Feb 10;16(2):e0246918. doi: 10.1371/journal.pone.0246918. eCollection 2021.
7
Essential Role for FtsL in Activation of Septal Peptidoglycan Synthesis.FtsL 在隔膜肽聚糖合成激活中的基本作用。
mBio. 2020 Dec 8;11(6):e03012-20. doi: 10.1128/mBio.03012-20.
8
Antibiotic susceptibility signatures identify potential antimicrobial targets in the Acinetobacter baumannii cell envelope.抗生素敏感性特征可鉴定鲍曼不动杆菌细胞包膜中的潜在抗菌靶标。
Nat Commun. 2020 Sep 9;11(1):4522. doi: 10.1038/s41467-020-18301-2.
9
A systematic review of implications, mechanisms, and stability of in vivo emergent resistance to colistin and tigecycline in .对 体内产生的多黏菌素和替加环素耐药性的影响、机制和稳定性的系统评价。
J Chemother. 2021 Feb;33(1):1-11. doi: 10.1080/1120009X.2020.1794393. Epub 2020 Jul 17.
10
The Science of Antibiotic Discovery.抗生素发现的科学。
Cell. 2020 Apr 2;181(1):29-45. doi: 10.1016/j.cell.2020.02.056. Epub 2020 Mar 19.