Xie Deng'e, Lu Chen, Zhu Ying, Zhu Sulan, Yang Er-Jiang, Jin Xin
Department of Urology, Shiyan Taihe Hospital, Affiliated Hospital to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
Department of Laboratory, Affiliated Hospital of Gannan Medical College, Ganzhou, Jiangxi 341000, P.R. China.
Exp Ther Med. 2018 Jun;15(6):5173-5176. doi: 10.3892/etm.2018.6115. Epub 2018 May 2.
We conducted this study to analyze the sperm DNA fragmentation index, conventional semen parameters, blood microelements and seminal plasma reactive oxygen species (ROS) in patients with male infertility to determine the association between each of the above male physiological parameters and DNA fragmentation index and infertility. Eighty cases of infertile males and 20 cases of normal males with children were divided into the infertility and control groups, respectively. Sperm DNA fragmentation index, conventional semen parameters, serum microelement content and seminal plasma ROS levels were detected, and the existing correlation between sperm DNA fragmentation index and the various physiological parameters were studied. The sperm DNA fragmentation index had no correlation with conventional sperm parameters. Our results demonstrated that zinc, lead and magnesium ions in the serum microelements were correlated with sperm DNA fragmentation (p<0.05). Upon an increase in zinc and lead serum concentration, there was a subsequent increase in sperm DNA fragmentation (p=0.008). Furthermore, when magnesium ion increased, it also caused an increase in sperm DNA fragmentation (p<0.05). The seminal plasma ROS of infertile males was higher than that of males with children (p<0.05). Our results suggest that sperm DNA fragmentation index is closely associated with the infertility rate and microelements of serum and seminal plasma ROS can impact the formation of sperm DNA fragmentation. Therefore, the sperm DNA fragmentation index can serve as an important parameter to assess male infertility.
我们开展这项研究，旨在分析男性不育患者的精子DNA碎片化指数、常规精液参数、血液微量元素以及精浆活性氧（ROS），以确定上述各项男性生理参数与DNA碎片化指数及不育之间的关联。80例不育男性患者和20例有子女的正常男性分别被分为不育组和对照组。检测精子DNA碎片化指数、常规精液参数、血清微量元素含量及精浆ROS水平，并研究精子DNA碎片化指数与各项生理参数之间的相关性。精子DNA碎片化指数与常规精子参数无相关性。我们的结果表明，血清微量元素中的锌、铅和镁离子与精子DNA碎片化相关（p<0.05）。血清锌和铅浓度升高时，精子DNA碎片化随之增加（p=0.008）。此外，镁离子增加时，也会导致精子DNA碎片化增加（p<0.05）。不育男性的精浆ROS高于有子女的男性（p<0.05）。我们的结果提示，精子DNA碎片化指数与不育率密切相关，血清微量元素及精浆ROS会影响精子DNA碎片化的形成。因此，精子DNA碎片化指数可作为评估男性不育的重要参数。
