Nanosensors and Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain.
Department of Chemistry in Pharmaceutical Sciences, Analytical Chemistry, Faculty of Pharmacy, Complutense University of Madrid, 28040, Madrid, Spain.
Mikrochim Acta. 2024 Oct 3;191(11):642. doi: 10.1007/s00604-024-06728-z.
The preparation of a hybrid nanomaterial is reported by covalently attaching 3,5-dinitrobenzoic acid groups to the surface of oxidized multi-walled carbon nanotubes using 1,6-diaminohexane as cross-linking agent. This nanomaterial, modified with the redox mediator, was used as transduction element to construct an amperometric sensor for the efficient indirect determination of glutathione reductase at a low working potential of - 0.05 V, through the oxidation of unconsumed nicotinamide adenine dinucleotide phosphate (NADPH) in the enzymatic reaction. The sensor exhibited an excellent linear response in the range 1.6 to 174 µU/µL, with high reproducibility and selectivity. The developed device was successfully validated in real samples, accurately determining the active enzyme in diluted human serum, making it a promising alternative for the determination of glutathione reductase and other related NADPH-dependent enzymes with relevance in clinical analysis.
通过使用 1,6-二氨基己烷作为交联剂，将 3,5-二硝基苯甲酸基团共价连接到氧化多壁碳纳米管表面，制备了一种杂化纳米材料。该修饰有氧化还原介体的纳米材料被用作换能元件，构建了一种安培传感器，可在低工作电位-0.05 V 下通过酶反应中未消耗的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的氧化，高效间接测定谷胱甘肽还原酶。该传感器在 1.6 至 174 µU/µL 的范围内表现出优异的线性响应，具有高重现性和选择性。所开发的设备在实际样品中得到了成功验证，可准确测定稀释人血清中的活性酶，为谷胱甘肽还原酶和其他相关 NADPH 依赖性酶的测定提供了有前途的替代方法，这些酶在临床分析中具有重要意义。
