Botany and Microbiology Department, Faculty of Science, Cairo University, Egypt.
Biophysics Department, Faculty of Science, Cairo University, Egypt.
Microb Pathog. 2024 Nov;196:106990. doi: 10.1016/j.micpath.2024.106990. Epub 2024 Oct 1.
The rising demand for innovative antimicrobial solutions has shifted focus towards silver nanoparticles (AgNPs), especially those produced through eco-friendly methods. This study introduces a novel approach utilizing actinomycetes strains-Streptomyces albus, Micromonospora maris, and Arthrobacter crystallopoietes-to biosynthesize AgNPs with remarkable antibacterial properties. Through molecular characterization, we identified unique features of these nanoparticles, and computational modeling suggested significant ion-ligand interactions with proteins 6REV and 3K07. Our research highlights the promise of these biogenically synthesized nanoparticles in advancing biomedical applications. Actinomycetes were sourced and screened for their ability to produce metallic nanoparticles, revealing that among 35 samples, only six showed this capability. Notably, Streptomyces albus strain smmdk14 (OR685674), Micromonospora maris strain smmdk13 (OR685672), and Arthrobacter crystallopoietes strain smmdk12 (OR685674) were identified as effective silver nanoparticle producers. The synthesized nanoparticles demonstrated potent antibacterial activity against common pathogens including E. coli, Pseudomonas aeruginosa, Klebsiella spp., Enterococcus faecalis, Staphylococcus aureus, and Acinetobacter spp. The data obtained from color change observation, UV-visible spectrophotometry, Zeta potential, FTIR spectroscopy, and transmission electron microscopy (TEM) characterized AgNPs potentiality. The nanoparticles were spherical, with sizes ranging from 6.46 nm to 24.7 nm. Optimization of production conditions, comparison of antimicrobial effects with antibiotics, evaluation of potential toxicity, and assessment of wound-healing capabilities were also conducted. The biosynthesized AgNPs exhibited superior antibacterial properties compared to traditional antibiotics and significantly accelerated wound healing by approximately 66.4 % in fibroblast cell cultures. Additionally, computational analysis predicted interactions between various metal ions and specific amino acid residues in proteins 6REV and 3K07. Overall, this study demonstrates the successful creation of AgNPs with notable antibacterial and wound-healing properties, underscoring their potential for medical applications.
对创新抗菌解决方案的需求不断增长，这使得人们将注意力转向了银纳米粒子（AgNPs），特别是那些通过环保方法制备的 AgNPs。本研究介绍了一种利用放线菌菌株——白色链霉菌、海洋微单胞菌和节杆菌——生物合成具有显著抗菌性能的 AgNPs 的新方法。通过分子特征分析，我们确定了这些纳米粒子的独特特征，并通过计算建模预测了它们与蛋白质 6REV 和 3K07 之间存在显著的离子-配体相互作用。我们的研究强调了这些生物合成纳米粒子在推进生物医学应用方面的潜力。从放线菌中筛选出能够生产金属纳米粒子的菌株，结果显示在 35 个样本中，只有 6 个具有这种能力。值得注意的是，白色链霉菌菌株 smmdk14（OR685674）、海洋微单胞菌菌株 smmdk13（OR685672）和节杆菌菌株 smmdk12（OR685674）被鉴定为有效的银纳米粒子生产者。合成的纳米粒子对包括大肠杆菌、铜绿假单胞菌、克雷伯氏菌属、粪肠球菌、金黄色葡萄球菌和不动杆菌属在内的常见病原体具有强大的抗菌活性。通过颜色变化观察、紫外可见分光光度法、Zeta 电位、傅里叶变换红外光谱和透射电子显微镜（TEM）等方法获得的数据对 AgNPs 的潜力进行了表征。纳米粒子呈球形，尺寸范围为 6.46nm 至 24.7nm。还进行了生产条件的优化、与抗生素的抗菌效果比较、潜在毒性评估以及伤口愈合能力评估。与传统抗生素相比，生物合成的 AgNPs 表现出优越的抗菌性能，并在成纤维细胞培养物中使伤口愈合速度显著加快了约 66.4%。此外，计算分析预测了各种金属离子与蛋白质 6REV 和 3K07 中特定氨基酸残基之间的相互作用。总的来说，本研究成功地制备了具有显著抗菌和伤口愈合性能的 AgNPs，突出了它们在医学应用方面的潜力。
