PG & Research Department of Microbiology, Sri Sankara Arts and Science College, Kanchipuram, Tamil Nadu, 631561, India.
Centre for Global Health Research, Saveetha Institute of Medical and Technical Sciences, Saveetha Medical College, Saveetha University, Chennai, India.
Mol Biol Rep. 2024 Jun 12;51(1):730. doi: 10.1007/s11033-024-09666-4.
Antimicrobial resistance has surged due to widespread antimicrobial drug use, prompting interest in biosynthesizing nanoparticles from marine-derived actinomycetes extracellular metabolites, valued for their diverse bioactive compounds. This approach holds promise for addressing the urgent need for novel antimicrobial agents. The current study aimed to characterize novel bioactive compounds from unexplored biodiversity hotspots, halophilic Streptomyces sp. isolated from mangrove sediment in the Pichavaram region, India.
Streptomyces rochei SSCM102 was conclusively identified through morphological and molecular characterization. Synthesis of silver nanoparticles (AgNPs) from Streptomyces rochei SSCM102 was characterized using various techniques, including UV-Vis, XRD, SEM, EDX, and FT-IR. The UV-Vis spectrum of the reduced AgNPs exhibited a prominent peak at 380 nm, confirming the AgNPs. The UV-Vis spectrum confirmed the synthesis of AgNP, and SEM analysis revealed a cubic morphology with sizes ranging from 11 to 21 nm. The FTIR spectrum demonstrated a shift in frequency widths between 626 cm and 3432 cm. The EDX analysis substantiated the presence of metallic silver, evident from a strong band at 1.44 keV. The synthesized AgNPs exhibited antibacterial efficacy against human pathogens Escherichia coli (64 ± 0.32 µg/ml), Klebsiella pneumoniae (32 ± 0.16 µg/ml), and Pseudomonas aeruginosa (16 ± 0.08 µg/ml) by MIC and MBC values of 128 ± 0.64 (µg/ml), 64 ± 0.32 (µg/ml) and 32 ± 0.16 (µg/ml), respectively. Additionally, at a concentration of 400 µg/ml, the AgNPs displayed a 72% inhibition of DPPH radicals, indicating notable antioxidant capacity. The LC value of 130 µg/mL indicates that the green-synthesized AgNPs have lower toxicity by Brine Shrimp Larvae assay.
The study's novel approach to synthesizing eco-friendly silver nanoparticles using Halophilic Streptomyces rochei SSCM102 contributes significantly to the field of biomedical research and drug development. By demonstrating potent antibacterial properties and aligning with sustainability goals, these nanoparticles offer promising avenues for novel antibacterial therapies.
由于广泛使用抗菌药物，抗菌药物耐药性急剧上升，这促使人们对从海洋来源放线菌的细胞外代谢产物中生物合成纳米颗粒产生了兴趣，这些纳米颗粒因其具有多样的生物活性化合物而备受重视。这种方法有望满足对新型抗菌剂的迫切需求。本研究旨在从印度皮查瓦拉姆地区红树林沉积物中分离的未开发生物多样性热点的嗜盐链霉菌中分离新型生物活性化合物。
通过形态学和分子鉴定，明确鉴定出罗奇氏链霉菌 SSCM102。采用各种技术，包括紫外-可见分光光度计、X 射线衍射、扫描电子显微镜、能谱仪和傅里叶变换红外光谱对罗奇氏链霉菌 SSCM102 合成的银纳米颗粒（AgNPs）进行了表征。还原的 AgNPs 的紫外-可见光谱在 380nm 处表现出明显的峰，证实了 AgNPs 的存在。紫外-可见光谱证实了 AgNP 的合成，扫描电子显微镜分析显示出 11 至 21nm 之间的立方形态。傅里叶变换红外光谱显示出 626cm 和 3432cm 之间的频率宽度的移动。能谱仪分析证实了金属银的存在，在 1.44keV 处有一个很强的带。合成的 AgNPs 通过 MIC 和 MBC 值对人类病原体大肠杆菌（64±0.32μg/ml）、肺炎克雷伯菌（32±0.16μg/ml）和铜绿假单胞菌（16±0.08μg/ml）表现出抗菌功效，分别为 128±0.64（μg/ml）、64±0.32（μg/ml）和 32±0.16（μg/ml）。此外，在 400μg/ml 的浓度下，AgNPs 对 DPPH 自由基的抑制率达到 72%，表明其具有显著的抗氧化能力。LC 值为 130μg/ml 表明，通过卤虫幼虫试验，绿色合成的 AgNPs 的毒性较低。
本研究采用嗜盐罗奇氏链霉菌 SSCM102 合成环保型银纳米颗粒的新方法，为生物医学研究和药物开发领域做出了重要贡献。这些纳米颗粒具有较强的抗菌性能，并与可持续性目标相一致，为新型抗菌疗法提供了有前途的途径。
