BGI Research, Shenzhen, China.
BGI Research, Hangzhou, China.
Methods Mol Biol. 2025;2850:417-434. doi: 10.1007/978-1-0716-4220-7_23.
Golden Gate Assembly (GGA) represents a versatile method for assembling multiple DNA fragments into a single molecule, which is widely used in rapid construction of complex expression cassettes for metabolic engineering. Here we describe the GGA method for facile construction and optimization of lycopene biosynthesis pathway by the combinatorial assembly of different transcriptional units (TUs). Furthermore, we report the method for characterizing and improving lycopene production in the synthetic yeast chassis.
金门装配（GGA）代表了一种将多个 DNA 片段组装成单个分子的通用方法，广泛用于代谢工程中复杂表达盒的快速构建。在这里，我们描述了通过不同转录单元（TU）的组合装配来轻松构建和优化番茄红素生物合成途径的 GGA 方法。此外，我们还报告了用于表征和提高合成酵母底盘中番茄红素产量的方法。
