Doughty M J
Am J Optom Physiol Opt. 1985 Dec;62(12):879-88. doi: 10.1097/00006324-198512000-00009.
Specular microscopy was used to measure thickness changes of stroma-endothelium preparations from rabbit corneas in vitro. The preparations were first bathed on both sides for 90 min with different bicarbonate-Ringer solutions (2 to 50 mM bicarbonate) maintained in equilibrium with 5% CO2-air (pH 6.2 to 7.9). During this equilibration, the stroma attained a stable thickness that was inversely related to the hydrostatic pressure (20 to 100 cm H2O) applied to the endothelial surface. After equilibration and at 20 cm H2O pressure, covering the anterior stromal surface with silicone oil (Dow Corning 200 dielectric fluid, 20 cs viscosity) resulted in stromal thinning. The rate of this deturgescence increased (from 5 to 75 micron/h) as the equilibration solution bicarbonate level increased from 2 to 30 mM. The net size of the thickness change was also related to the equilibration bicarbonate level. However, indirect studies on the cornea using phenol red indicator and pH electrode measurements of solutions revealed that the stromal bathing solutions became more alkaline under the silicone oil layer. CO2 is soluble in silicone oil. Implications of these CO2 and pH effects on mechanisms of corneal deturgescence are discussed.
利用镜面显微镜在体外测量兔眼角膜基质 - 内皮制剂的厚度变化。首先将制剂两侧用不同碳酸氢盐 - 林格氏溶液（2至50 mM碳酸氢盐）浸泡90分钟，这些溶液与5% CO₂ - 空气保持平衡（pH值6.2至7.9）。在这个平衡过程中，基质达到一个稳定的厚度，该厚度与施加在内皮表面的静水压力（20至100 cm H₂O）呈反比。平衡后，在20 cm H₂O压力下，用硅油（道康宁200介电液，粘度20 cs）覆盖前基质表面会导致基质变薄。随着平衡溶液中碳酸氢盐水平从2 mM增加到30 mM，这种消肿速率增加（从5到75微米/小时）。厚度变化的净大小也与平衡碳酸氢盐水平有关。然而，使用酚红指示剂和溶液pH电极测量对角膜进行的间接研究表明，在硅油层下基质浸泡溶液变得更碱性。CO₂可溶于硅油。讨论了这些CO₂和pH效应对角膜消肿机制的影响。
