在铜离子和硫醇化合物存在的情况下，羟基自由基的位点特异性和体相生成。
Site-specific and bulk-phase generation of hydroxyl radicals in the presence of cupric ions and thiol compounds.
作者信息
van Steveninck J, van der Zee J, Dubbelman T M
出版信息
Biochem J. 1985 Nov 15;232(1):309-11. doi: 10.1042/bj2320309.
Abstract
Addition of a thiol compound to a solution containing Cu2+ and H2O2 resulted in the generation of hydroxyl radicals (OH.). These radicals were able to oxidize salicylic acid and tryptamine in a reaction that was strongly inhibited by the OH.-scavenger mannitol. Covalent coupling of the thiol compound to tryptamine did not significantly influence the degradation of the indole moiety subsequent to addition of H2O2 and Cu2+. The inhibiting effect of mannitol, however, was strongly reduced, indicating that the scavenger could not interfere with site-specific reactions of OH..
摘要
向含有Cu2+和H2O2的溶液中添加硫醇化合物会导致羟基自由基（OH.）的生成。这些自由基能够在一个被OH-清除剂甘露醇强烈抑制的反应中氧化水杨酸和色胺。硫醇化合物与色胺的共价偶联在添加H2O2和Cu2+后对吲哚部分的降解没有显著影响。然而，甘露醇的抑制作用大大降低，这表明该清除剂无法干扰OH.的位点特异性反应。
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本文引用的文献
1
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2
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FEBS Lett. 1982 Feb 8;138(1):33-6. doi: 10.1016/0014-5793(82)80388-8.
3
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Biochem J. 1984 Aug 15;222(1):1-15. doi: 10.1042/bj2220001.
4
Oxygen toxicity, oxygen radicals, transition metals and disease.氧中毒、氧自由基、过渡金属与疾病。
Biochem J. 1984 Apr 1;219(1):1-14. doi: 10.1042/bj2190001.
5
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J Biol Chem. 1984 May 10;259(9):5606-11.
6
The production of free radicals during the autoxidation of cysteine and their effect on isolated rat hepatocytes.半胱氨酸自氧化过程中自由基的产生及其对分离的大鼠肝细胞的影响。
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7
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8
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