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在铜离子和硫醇化合物存在的情况下，羟基自由基的位点特异性和体相生成。

Site-specific and bulk-phase generation of hydroxyl radicals in the presence of cupric ions and thiol compounds.

作者信息

van Steveninck J, van der Zee J, Dubbelman T M

出版信息

Biochem J. 1985 Nov 15;232(1):309-11. doi: 10.1042/bj2320309.

DOI:10.1042/bj2320309
PMID:3936485
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152878/
Abstract

Addition of a thiol compound to a solution containing Cu2+ and H2O2 resulted in the generation of hydroxyl radicals (OH.). These radicals were able to oxidize salicylic acid and tryptamine in a reaction that was strongly inhibited by the OH.-scavenger mannitol. Covalent coupling of the thiol compound to tryptamine did not significantly influence the degradation of the indole moiety subsequent to addition of H2O2 and Cu2+. The inhibiting effect of mannitol, however, was strongly reduced, indicating that the scavenger could not interfere with site-specific reactions of OH..

摘要

向含有Cu2+和H2O2的溶液中添加硫醇化合物会导致羟基自由基（OH.）的生成。这些自由基能够在一个被OH-清除剂甘露醇强烈抑制的反应中氧化水杨酸和色胺。硫醇化合物与色胺的共价偶联在添加H2O2和Cu2+后对吲哚部分的降解没有显著影响。然而，甘露醇的抑制作用大大降低，这表明该清除剂无法干扰OH.的位点特异性反应。

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本文引用的文献

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Copper salt-dependent hydroxyl radical formation. Damage to proteins acting as antioxidants.铜盐依赖性羟基自由基的形成。对抗氧化蛋白的损伤。
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Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
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Generation of superoxide free radical during the autoxidation of thiols.硫醇自氧化过程中超氧自由基的产生。
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