Atakisi Emine, Atakisi Onur, Ozturkler Melek, Dalginli Kezban Yildiz, Ozbey Cagatay
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Departments of Chemistry, Faculty of Science and Letter, Kafkas University, Kars, Turkey.
Biol Trace Elem Res. 2024 Oct 4. doi: 10.1007/s12011-024-04402-2.
Aluminum (Al), one of the three most prevalent metals in the Earth's crust, adversely impacts all metabolic systems of living organisms due to its extensive utilization by humans. It is known that omega-3 fatty acids (ω-3FA) protect the organism against diseases and have positive effects on the immune system. The aim of the study was to investigate the effect of ω-3FA on 8-OH-2-deoxyguanosine (8-OHdG), glutathione (GSH) levels and adenosine deaminase (ADA), paraoxonase (PON), and catalase (CAT) activities in rats with acute aluminum toxicity. The study also aimed to investigate the antioxidant system, as well as Al, zinc (Zn), and iron (Fe) levels. Forty Sprague-Dawley rats (n = 40) were used in the study and the rats were divided into four equal groups (n = 10). In group I, 0.5 mL of 0.9% saline solution (NaCI) was injected intraperitoneally. Group II was injected with 34 mg/kg aluminum chloride (AlCI) intraperitoneally. Group III received 400 mg/kg ω-3FA for 7 days and group IV received both AlCI and 400 mg/kg ω-3FA for 7 days. At the end of the study, blood samples were obtained by cardiac puncture. The findings showed that Al exposure increased serum 8-OHdG and total oxidant status (TOS) levels, as well as ADA activity, which are markers associated with oxidative damage. Conversely, PON and CAT activities, GSH, and total antioxidant status (TAS) levels decreased compared to the control group. Furthermore, Zn and Fe levels decreased as Al levels increased. In conclusion, Al has the capacity to induce oxidative damage and lipid peroxidation, while ω-3 fatty acids may mitigate this damage through a regulatory mechanism. Moreover, ω-3-FA could be used as a therapeutic agent that reduces Al toxicity.
铝（Al）是地壳中三种最常见的金属之一，由于人类对其广泛使用，它会对生物体的所有代谢系统产生不利影响。已知ω-3脂肪酸（ω-3FA）能保护机体免受疾病侵害，并对免疫系统产生积极影响。本研究的目的是调查ω-3FA对急性铝中毒大鼠体内8-羟基-2-脱氧鸟苷（8-OHdG）、谷胱甘肽（GSH）水平以及腺苷脱氨酶（ADA）、对氧磷酶（PON）和过氧化氢酶（CAT）活性的影响。该研究还旨在调查抗氧化系统以及铝、锌（Zn）和铁（Fe）的水平。本研究使用了40只Sprague-Dawley大鼠（n = 40），并将大鼠分为四个相等的组（n = 10）。第一组腹腔注射0.5 mL 0.9%的生理盐水（NaCl）。第二组腹腔注射34 mg/kg的氯化铝（AlCl）。第三组连续7天接受400 mg/kg的ω-3FA，第四组连续7天同时接受AlCl和400 mg/kg的ω-3FA。在研究结束时，通过心脏穿刺采集血样。研究结果表明，铝暴露会增加血清8-OHdG和总氧化状态（TOS）水平以及ADA活性，这些都是与氧化损伤相关的标志物。相反，与对照组相比，PON和CAT活性、GSH以及总抗氧化状态（TAS）水平降低。此外，随着铝水平的升高，锌和铁水平降低。总之，铝有能力诱导氧化损伤和脂质过氧化，而ω-3脂肪酸可能通过调节机制减轻这种损伤。此外，ω-3-FA可作为一种降低铝毒性的治疗剂。
