Müller R, Poch O, Delarue M, Bishop D H, Bouloy M
Laboratoire des Bunyaviridés, CNRS URA 545, Institut Pasteur, Paris, France.
J Gen Virol. 1994 Jun;75 ( Pt 6):1345-52. doi: 10.1099/0022-1317-75-6-1345.
The sequence of Rift Valley fever virus L segment that we published in a previous paper was erroneous in the 3'-terminal region of the antigenomic RNA molecule. Here, we have shown that the L segment is in fact 6404 nucleotides long and encodes a polypeptide of 237.7K in the viral complementary sense. Sequence comparisons performed between the RNA-dependent RNA polymerases of 22 negative-stranded RNA viruses revealed the existence of two novel regions located at the amino termini of the proteins and conserved only in the polymerases of bunya- and arenaviruses. In the region conserved in all RNA-dependent polymerases, corresponding to the so-called 'polymerase module', we identified a new motif, designated premotif A, common to all RNA-dependent polymerases, as well as amino acids located in the region between motifs preA and A which are strictly conserved for segmented negative-stranded RNA viruses. Using the recently released coordinates of human immunodeficiency virus reverse transcriptase and the alignment between all RNA-dependent polymerases in the 'polymerase module', we have determined the position of the conserved residues in these polymerases and discuss their possible functions in light of the available structural information.
我们在前一篇论文中发表的裂谷热病毒L片段序列在反基因组RNA分子的3'末端区域存在错误。在此，我们已表明L片段实际上长6404个核苷酸，并以病毒互补链编码一个237.7K的多肽。对22种负链RNA病毒的RNA依赖性RNA聚合酶进行的序列比较揭示了位于蛋白质氨基末端的两个新区域，且仅在布尼亚病毒科和沙粒病毒科的聚合酶中保守。在所有RNA依赖性聚合酶保守的区域，即对应于所谓的“聚合酶模块”中，我们鉴定出一个新基序，命名为前基序A，它存在于所有RNA依赖性聚合酶中，以及位于前基序A和基序A之间区域的氨基酸，这些氨基酸对于分节段负链RNA病毒是严格保守的。利用最近公布的人类免疫缺陷病毒逆转录酶的坐标以及“聚合酶模块”中所有RNA依赖性聚合酶之间的比对，我们确定了这些聚合酶中保守残基的位置，并根据现有的结构信息讨论了它们可能的功能。
