Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; School of Life Sciences, South China Normal University, Guangzhou 510631, China.
School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Cell Rep. 2024 Oct 22;43(10):114798. doi: 10.1016/j.celrep.2024.114798. Epub 2024 Oct 3.
It has been widely recognized that the microbiota has the capacity to shape host gene expression and physiological functions. However, there remains a paucity of comprehensive study revealing the host transcriptional landscape regulated by the microbiota. Here, we comprehensively examined mRNA landscapes in mouse tissues (brain and cecum) from specific-pathogen-free and germ-free mice using nanopore direct RNA sequencing. Our results show that the microbiome has global influence on a host's RNA modifications (mA, mC, Ψ), isoform generation, poly(A) tail length, and transcript abundance in both brain and cecum tissues. Moreover, the microbiome exerts tissue-specific effects on various post-transcriptional regulatory processes. In addition, the microbiome impacts the coordination of multiple RNA modifications in host brain and cecum tissues. In conclusion, we establish the relationship between microbial regulation and gene expression. Our results help the understanding of the mechanisms by which the microbiome reprograms host gene expression.
人们已经广泛认识到，微生物组具有塑造宿主基因表达和生理功能的能力。然而，目前仍缺乏全面的研究来揭示微生物组调控宿主转录组的全貌。在这里，我们使用纳米孔直接 RNA 测序，全面检查了无特定病原体和无菌小鼠的鼠组织（大脑和盲肠）中的 mRNA 图谱。我们的结果表明，微生物组对宿主的 RNA 修饰（mA、mC、Ψ）、异构体生成、多聚（A）尾长和大脑和盲肠组织中转录物丰度具有全局影响。此外，微生物组对各种转录后调控过程具有组织特异性影响。此外，微生物组影响宿主大脑和盲肠组织中多个 RNA 修饰的协调。总之，我们建立了微生物调节与基因表达之间的关系。我们的研究结果有助于理解微生物组重新编程宿主基因表达的机制。
