Huang Sihao, Wylder Adam C, Pan Tao
Department of Biochemistry & Molecular Biology, University of Chicago, Chicago, IL, USA.
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.
Nat Biotechnol. 2024 Dec;42(12):1831-1835. doi: 10.1038/s41587-024-02135-0. Epub 2024 Feb 6.
N6-methyladenosine (mA) and pseudouridine (Ψ) are the two most abundant modifications in mammalian messenger RNA, but the coordination of their biological functions remains poorly understood. We develop a machine learning-based nanopore direct RNA sequencing method (NanoSPA) that simultaneously analyzes mA and Ψ in the human transcriptome. Applying NanoSPA to polysome profiling, we reveal opposing transcriptomic co-occurrence of mA and Ψ and synergistic, hierarchical effects of mA and Ψ on the polysome.
N6-甲基腺苷(mA)和假尿苷(Ψ)是哺乳动物信使核糖核酸中两种含量最丰富的修饰,但对它们生物学功能的协同作用仍知之甚少。我们开发了一种基于机器学习的纳米孔直接RNA测序方法(NanoSPA),可同时分析人类转录组中的mA和Ψ。将NanoSPA应用于多核糖体分析,我们揭示了mA和Ψ在转录组中的相反共现情况,以及mA和Ψ对多核糖体的协同、分级效应。