• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

模拟热灭活粘质沙雷氏菌（Serratia marcescens）对蜜蜂（Apis mellifera L.）注射后的季节性免疫动态。

Modeling seasonal immune dynamics of honey bee (Apis mellifera L.) response to injection of heat-killed Serratia marcescens.

机构信息

Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.

Department of Mathematical Analysis and Application of Mathematics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic.

出版信息

PLoS One. 2024 Oct 4;19(10):e0311415. doi: 10.1371/journal.pone.0311415. eCollection 2024.

DOI:10.1371/journal.pone.0311415
PMID:39365765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11452037/
Abstract

The honey bee, Apis mellifera L., is one of the main pollinators worldwide. In a temperate climate, seasonality affects the life span, behavior, physiology, and immunity of honey bees. In consequence, it impacts their interaction with pathogens and parasites. In this study, we used Bayesian statistics and modeling to examine the immune response dynamics of summer and winter honey bee workers after injection with the heat-killed bacteria Serratia marcescens, an opportunistic honey bee pathogen. We investigated the humoral and cellular immune response at the transcriptional and functional levels using qPCR of selected immune genes, antimicrobial activity assay, and flow cytometric analysis of hemocyte concentration. Our data demonstrate increased antimicrobial activity at transcriptional and functional levels in summer and winter workers after injection, with a stronger immune response in winter bees. On the other hand, an increase in hemocyte concentration was observed only in the summer bee population. Our results indicate that the summer population mounts a cellular response when challenged with heat-killed S. marcescens, while winter honey bees predominantly rely on humoral immune reactions. We created a model describing the honey bee immune response dynamics to bacteria-derived components by applying Bayesian statistics to our data. This model can be employed in further research and facilitate the investigating of the honey bee immune system and its response to pathogens.

摘要

蜜蜂，Apis mellifera L.，是世界上主要的传粉媒介之一。在温带气候中，季节性会影响蜜蜂的寿命、行为、生理和免疫力。因此，它会影响它们与病原体和寄生虫的相互作用。在这项研究中，我们使用贝叶斯统计和建模来研究夏季和冬季工蜂在注射热灭活细菌粘质沙雷氏菌（一种机会性蜜蜂病原体）后的免疫反应动态。我们使用选定免疫基因的 qPCR、抗菌活性测定和血细胞浓度的流式细胞分析，在转录和功能水平上研究了体液和细胞免疫反应。我们的数据表明，夏季和冬季工蜂在注射后，在转录和功能水平上的抗菌活性增加，冬季蜜蜂的免疫反应更强。另一方面，仅在夏季蜂群中观察到血细胞浓度增加。我们的结果表明，夏季蜂群在受到热灭活 S. marcescens 挑战时会产生细胞反应，而冬季蜜蜂主要依赖体液免疫反应。我们通过将贝叶斯统计应用于我们的数据，创建了一个描述蜜蜂对细菌衍生成分的免疫反应动态的模型。该模型可用于进一步的研究，并有助于研究蜜蜂的免疫系统及其对病原体的反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/5f332e2141d2/pone.0311415.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/b1c5ebb2f396/pone.0311415.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/d101ee0af4df/pone.0311415.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/e401cb561c48/pone.0311415.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/6b97e0803edf/pone.0311415.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/e3fce684fa11/pone.0311415.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/3229fab0342d/pone.0311415.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/8c6cd0957c3a/pone.0311415.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/5f332e2141d2/pone.0311415.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/b1c5ebb2f396/pone.0311415.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/d101ee0af4df/pone.0311415.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/e401cb561c48/pone.0311415.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/6b97e0803edf/pone.0311415.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/e3fce684fa11/pone.0311415.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/3229fab0342d/pone.0311415.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/8c6cd0957c3a/pone.0311415.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/75c5/11452037/5f332e2141d2/pone.0311415.g008.jpg

相似文献

1
Modeling seasonal immune dynamics of honey bee (Apis mellifera L.) response to injection of heat-killed Serratia marcescens.模拟热灭活粘质沙雷氏菌（Serratia marcescens）对蜜蜂（Apis mellifera L.）注射后的季节性免疫动态。
PLoS One. 2024 Oct 4;19(10):e0311415. doi: 10.1371/journal.pone.0311415. eCollection 2024.
2
Pathogenicity of Serratia marcescens Strains in Honey Bees.蜂群中粘质沙雷氏菌的致病性。
mBio. 2018 Oct 9;9(5):e01649-18. doi: 10.1128/mBio.01649-18.
3
Sepsis and Hemocyte Loss in Honey Bees (Apis mellifera) Infected with Serratia marcescens Strain Sicaria.感染粘质沙雷氏菌菌株Sicaria的蜜蜂（西方蜜蜂）的败血症和血细胞损失
PLoS One. 2016 Dec 21;11(12):e0167752. doi: 10.1371/journal.pone.0167752. eCollection 2016.
4
Overwintering Is Associated with Reduced Expression of Immune Genes and Higher Susceptibility to Virus Infection in Honey Bees.越冬与蜜蜂免疫基因表达降低及对病毒感染的易感性增加有关。
PLoS One. 2015 Jun 29;10(6):e0129956. doi: 10.1371/journal.pone.0129956. eCollection 2015.
5
Hemocyte-mediated phagocytosis differs between honey bee (Apis mellifera) worker castes.血细胞介导的吞噬作用在蜜蜂（西方蜜蜂）工蜂等级之间存在差异。
PLoS One. 2017 Sep 6;12(9):e0184108. doi: 10.1371/journal.pone.0184108. eCollection 2017.
6
Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes.鉴定用于描述蜜蜂（Apis mellifera）血淋巴细胞类别的参考标记物。
Dev Comp Immunol. 2020 Aug;109:103701. doi: 10.1016/j.dci.2020.103701. Epub 2020 Apr 19.
7
Silencing the Honey Bee (Apis mellifera) Naked Cuticle Gene (nkd) Improves Host Immune Function and Reduces Nosema ceranae Infections.沉默蜜蜂（西方蜜蜂）的裸表皮基因（nkd）可改善宿主免疫功能并减少蜜蜂微孢子虫感染。
Appl Environ Microbiol. 2016 Oct 27;82(22):6779-6787. doi: 10.1128/AEM.02105-16. Print 2016 Nov 15.
8
The Gut Microbiota Protects Bees from Invasion by a Bacterial Pathogen.肠道微生物群保护蜜蜂免受细菌病原体的侵袭。
Microbiol Spectr. 2021 Oct 31;9(2):e0039421. doi: 10.1128/Spectrum.00394-21. Epub 2021 Sep 15.
9
Seasonal Dynamics of the Honey Bee Gut Microbiota in Colonies Under Subtropical Climate : Seasonal Dynamics of Honey Bee Gut Microbiota.亚热带气候条件下蜂群肠道微生物组的季节性动态： 蜜蜂肠道微生物组的季节性动态。
Microb Ecol. 2022 Feb;83(2):492-500. doi: 10.1007/s00248-021-01756-1. Epub 2021 May 11.
10
Community Dynamics in Structure and Function of Honey Bee Gut Bacteria in Response to Winter Dietary Shift.蜜蜂肠道细菌结构和功能的社区动态对冬季饮食转变的响应。
mBio. 2022 Oct 26;13(5):e0113122. doi: 10.1128/mbio.01131-22. Epub 2022 Aug 29.

本文引用的文献

1
can promote development in honeybee gut: an underrated threat for bees?可以促进蜜蜂肠道的发育：这对蜜蜂来说是一个被低估的威胁吗？
Front Cell Infect Microbiol. 2024 May 13;14:1323157. doi: 10.3389/fcimb.2024.1323157. eCollection 2024.
2
Chronic exposure to pesticides disrupts the bacterial and fungal co-existence and the cross-kingdom network characteristics of honey bee gut microbiome.长期接触农药会破坏蜜蜂肠道微生物组中细菌和真菌的共存和跨界网络特征。
Sci Total Environ. 2024 Jan 1;906:167530. doi: 10.1016/j.scitotenv.2023.167530. Epub 2023 Oct 11.
3
Varroa destructor.
狄斯瓦螨
Trends Parasitol. 2023 Jun;39(6):487-488. doi: 10.1016/j.pt.2023.03.009. Epub 2023 Mar 24.
4
Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation.基于组学的蜜蜂（西方蜜蜂）对瓦螨属寄生反应及相关因素的分析揭示了损害越冬蜂产生的变化。
Insect Biochem Mol Biol. 2023 Jan;152:103877. doi: 10.1016/j.ibmb.2022.103877. Epub 2022 Nov 18.
5
Bees under interactive stressors: the novel insecticides flupyradifurone and sulfoxaflor along with the fungicide azoxystrobin disrupt the gut microbiota of honey bees and increase opportunistic bacterial pathogens.受交互胁迫影响的蜜蜂：新型杀虫剂氟氯虫酰胺和噻虫胺，以及杀菌剂唑菌胺酯，会破坏蜜蜂的肠道微生物群，并增加机会性细菌病原体。
Sci Total Environ. 2022 Nov 25;849:157941. doi: 10.1016/j.scitotenv.2022.157941. Epub 2022 Aug 9.
6
Winter honeybee () populations show greater potential to induce immune responses than summer populations after immune stimuli.冬季蜂群（）在免疫刺激后比夏季蜂群更能诱导免疫反应。
J Exp Biol. 2021 Feb 8;224(Pt 3):jeb232595. doi: 10.1242/jeb.232595.
7
Parasite defense mechanisms in bees: behavior, immunity, antimicrobials, and symbionts.蜜蜂的寄生虫防御机制：行为、免疫、抗菌物质和共生体。
Emerg Top Life Sci. 2020 Jul 2;4(1):59-76. doi: 10.1042/ETLS20190069.
8
Varroa destructor: A Complex Parasite, Crippling Honey Bees Worldwide.瓦螨：一种复杂的寄生虫，正在使全球蜜蜂陷入瘫痪。
Trends Parasitol. 2020 Jul;36(7):592-606. doi: 10.1016/j.pt.2020.04.004. Epub 2020 May 23.
9
The Year of the Honey Bee ( L.) with Respect to Its Physiology and Immunity: A Search for Biochemical Markers of Longevity.意蜂（L.）的生理与免疫之年：探寻长寿的生化标志物
Insects. 2019 Aug 7;10(8):244. doi: 10.3390/insects10080244.
10
Immunosenescence in honey bees (Apis mellifera L.) is caused by intrinsic senescence and behavioral physiology.蜜蜂（Apis mellifera L.）的免疫衰老由内在衰老和行为生理学引起。
Exp Gerontol. 2019 May;119:174-183. doi: 10.1016/j.exger.2019.02.005. Epub 2019 Feb 8.