Artificial Cells and Organs Research Centre, Departments of Phsyiology, Medicine and Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, Canada.
Sci Rep. 2024 Oct 4;14(1):23104. doi: 10.1038/s41598-024-73862-2.
This study investigates the potential improvement of polyhemoglobin's protective properties by the addition of 3 synthetic enzymes (neo-carbonic anhydrase, neo-catalase and neo-superoxide dismutase) to polyhemoglobin after 90 and 180 min of warm in-vitro ischemia (100% Nitrogen at 37 °C). Following the warm ischemic shock, cell cultures were subjected to various treatment solutions: Controls; PolyHb; 3 neoenzymes; PolyHb + 3 neoenzymes; PolyHb + 2 neoenzymes. The cultures were then incubated (Oxygen, 5% CO at 37 °C) for 24 h followed by several analyses. Compared to polyhemoglobin alone, this novel solution containing polyhemoglobin + 3 neoeznymes significantly increased the viability, cell growth, albumin production, protection against oxidative stress and cellular injury of human hepatocytes. Moreover, this also protects the viability of human cardiomyocytes. These findings suggest that it could be useful as a pre-transplant cell/organ preservation solution which, in the long-term, could contribute to the development of blood substitutes.
本研究探讨了在 37°C 的 100%氮气中进行 90 和 180 分钟温热体外缺血后，向聚血红蛋白中添加 3 种合成酶（新碳酸酐酶、新过氧化氢酶和新超氧化物歧化酶）对聚血红蛋白的保护性能的潜在改善。在温热缺血性休克后，细胞培养物接受了各种处理溶液：对照；聚血红蛋白；3 种新酶；聚血红蛋白+3 种新酶；聚血红蛋白+2 种新酶。然后将培养物在（氧气，5% CO，37°C）下孵育 24 小时，然后进行多项分析。与单独的聚血红蛋白相比，这种含有聚血红蛋白+3 种新酶的新型溶液显著提高了人肝细胞的活力、细胞生长、白蛋白产生、抗氧化应激和细胞损伤的保护作用。此外，它还可以保护人心肌细胞的活力。这些发现表明，它可用作移植前细胞/器官保存溶液，从长远来看，这可能有助于血液替代品的开发。
