Department of Genetics, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Funct Integr Genomics. 2024 Oct 5;24(5):183. doi: 10.1007/s10142-024-01421-z.
In personalized cancer medicine, the identification of KRAS mutations is essential for making treatment decisions and improving patient outcomes. This work presents a comprehensive review of the current approaches for detection of KRAS mutations in different cancers. We highlight the value of fast and reliable KRAS mutations discovery and the effectiveness of molecular testing for selecting individuals who might benefit from targeted therapy. We provide an overview of various methods and tools available for detecting KRAS mutations, such as digital droplet PCR, next-generation sequencing (NGS), and polymerase chain reaction (PCR). We also address the difficulties and limitations in the identification of KRAS mutations, namely tumor heterogeneity and the emergence of resistance mechanisms. This article aims to guide clinicians in KRAS mutation identification.
在个性化癌症医学中，鉴定 KRAS 突变对于做出治疗决策和改善患者预后至关重要。本工作全面综述了目前用于不同癌症中 KRAS 突变检测的方法。我们强调了快速可靠的 KRAS 突变发现的价值，以及分子检测在选择可能受益于靶向治疗的个体方面的有效性。我们提供了用于检测 KRAS 突变的各种方法和工具的概述，例如数字液滴 PCR、下一代测序 (NGS) 和聚合酶链反应 (PCR)。我们还讨论了鉴定 KRAS 突变时面临的困难和局限性，即肿瘤异质性和耐药机制的出现。本文旨在为临床医生提供 KRAS 突变鉴定方面的指导。
