鹧鸪（石鸡）的抗体反应 1. 性别和年龄对绵羊红细胞（SRBC）、新城疫病毒（NDV）和流产布鲁氏菌（Buck 19）免疫反应的影响

Antibody response in partridge (Perdix perdix L.) 1. Effect of sex and age on the immune response to sheep red blood cells (SRBC), Newcastle disease virus (NDV) and Brucella abortus (Buck 19).

作者信息

Randi E, Chiricolo M, Spagnesi M, Ghedini I, Savigni G, Giovannini A, Franceschi C

出版信息

Dev Comp Immunol. 1985 Fall;9(4):679-90. doi: 10.1016/0145-305x(85)90032-1.

Abstract

To ascertain the immune response capacity of Partridges to classical experimental and natural antigens, dose and time response curves to sheep red blood cells (SRBC), Newcastle disease virus (NDV) and Brucella abortus (Buck 19 strain), were determined in two strains reared in two different farms. 1 ml of 20% SRBC, 10(9) NDV particles and 10(7) Buck 19 microorganisms, gave optimal antibody responses seven (SRBC and Buck 19) or fourteen days after immunization. As far as SRBC is concerned, the antibody response was sex independent, but declined markedly in partridges older than 1 year. The immunization schedule used by us allow the identification of high and low responder animals. In fact, highly significant correlation in individual partridges between antibody titers obtained after two successive immunizations to an optimal SRBC dose given three weeks apart, was found. This fact, together with the observed great variability in the antibody response to the three antigens considered, should allow a selection for high and low responder lines, in order to improve the fitness toward environmental pathogens.

摘要

为确定鹧鸪对经典实验性抗原和天然抗原的免疫反应能力，在两个不同农场饲养的两个品系鹧鸪中，测定了对绵羊红细胞（SRBC）、新城疫病毒（NDV）和流产布鲁氏菌（巴克19株）的剂量反应曲线和时间反应曲线。1毫升20%的SRBC、10⁹个NDV颗粒和10⁷个巴克19微生物，在免疫后7天（SRBC和巴克19）或14天产生最佳抗体反应。就SRBC而言，抗体反应与性别无关，但在1岁以上的鹧鸪中明显下降。我们采用的免疫程序能够识别高反应和低反应动物。事实上，在个体鹧鸪中，发现相隔三周给予最佳SRBC剂量后连续两次免疫所获得的抗体滴度之间存在高度显著的相关性。这一事实，连同观察到的对所考虑的三种抗原的抗体反应存在很大变异性，应该能够选择高反应和低反应品系，以提高对环境病原体的适应性。