Bhanushali J K, Murthy K K, Ragland W L
Dev Comp Immunol. 1985 Summer;9(3):453-63. doi: 10.1016/0145-305x(85)90008-4.
In order to determine if mibolerone causes rapid development of the humoral immune system as previously hypothesized, 12-day-old chicken embryos were injected with 0.1, 1, or 10 micrograms mibolerone. Mibolerone caused bursal atrophy in a dose-dependent manner with severe atrophy at the 10 micrograms dose, substantial atrophy at the 1 microgram dose, and very little or no atrophy at the 0.1 microgram dose. Mibolerone-treated chicks were subsequently challenged with sheep red blood cells (SRBC) and Brucella abortus at 1, 7, 14, or 21 days of age. When serum samples were analysed for agglutinins to SRBC and B. abortus, the results indicated that treated chickens had lower agglutinin titers to both antigens at younger ages. These responses improved with age in groups treated with 0.1 microgram and 1 microgram and were comparable to controls at 5 weeks of age except for low primary and secondary (IgG specific) titers to B. abortus in birds treated with 1 microgram. Chickens treated with 10 micrograms mibolerone still had considerably lower primary responses to SRBC and lower primary as well as secondary responses to B. abortus at 4 and 5 weeks of age. These results indicate that mibolerone causes delayed rather than rapid development of the humoral immune system. In control as well as in treated chickens, agglutinin responses to SRBC appeared earlier than responses to B. abortus.
为了确定米勃酮是否如先前假设的那样会导致体液免疫系统快速发育，给12日龄的鸡胚注射了0.1微克、1微克或10微克的米勃酮。米勃酮呈剂量依赖性地导致法氏囊萎缩，10微克剂量时出现严重萎缩，1微克剂量时出现明显萎缩，0.1微克剂量时萎缩很少或没有萎缩。随后，在1日龄、7日龄、14日龄或21日龄时，用绵羊红细胞（SRBC）和流产布鲁氏菌对经米勃酮处理的雏鸡进行攻毒。当分析血清样本中针对SRBC和流产布鲁氏菌的凝集素时，结果表明，在较年轻时，经处理的鸡对两种抗原的凝集素滴度较低。在接受0.1微克和1微克处理的组中，这些反应随年龄增长而改善，除了接受1微克处理的鸡对流产布鲁氏菌的初次和二次（IgG特异性）滴度较低外，在5周龄时与对照组相当。接受10微克米勃酮处理的鸡在4周龄和5周龄时对SRBC的初次反应仍然相当低，对流产布鲁氏菌的初次和二次反应也较低。这些结果表明，米勃酮导致体液免疫系统发育延迟而非快速发育。在对照鸡和经处理的鸡中，对SRBC的凝集素反应比对流产布鲁氏菌的反应出现得更早。
