van der Zijpp A J, Rooyakkers J M, Kouwenhoven B
Avian Dis. 1982 Jan-Mar;26(1):97-106.
The effects of viral vaccinations and immunization with sheep red blood cells (SRBC) on the humoral response of pullets were investigated. Pullets were vaccinated with Marek's disease virus, Newcastle disease virus (NDV), infectious bronchitis virus (IBV), and infectious bursal disease virus at appropriate ages used in commercial practice. At seven weeks, the pullets were intramuscularly immunized with SRBC. NDV and IBV antibodies were detected by hemagglutination-inhibition tests. Hemagglutination (HA) titers were established against SRBC. IBV antibody titers were not affected by vaccination or by immunization with SRBC. NDV antibody titers were significantly increased by vaccination and by immunization with SRBC. The SRBC agglutinin response was also positively affected by vaccination. The HA titer increase consisted of a rise in 2-mercaptoethanol (2-ME)-sensitive antibodies and a fall in 2-ME-resistant antibodies.
研究了病毒疫苗接种以及用绵羊红细胞（SRBC）免疫对小母鸡体液反应的影响。在商业实践中，在适当的年龄给小母鸡接种马立克氏病病毒、新城疫病毒（NDV）、传染性支气管炎病毒（IBV）和传染性法氏囊病病毒。在7周龄时，给小母鸡肌肉注射SRBC进行免疫。通过血凝抑制试验检测NDV和IBV抗体。建立了针对SRBC的血凝（HA）效价。IBV抗体效价不受疫苗接种或SRBC免疫的影响。接种疫苗和用SRBC免疫均显著提高了NDV抗体效价。疫苗接种也对SRBC凝集素反应产生了积极影响。HA效价的增加包括2-巯基乙醇（2-ME）敏感抗体的增加和2-ME抗性抗体的减少。
