Wang Longyun, Wang Mengqi, Wang Zeyu, Wang Kai, Zhao Bowei, Wang Yue, Zheng Jingying, Zhang Shuang
Department of Rehabilitation, School of Nursing, Jilin University, Changchun, 130021, Jilin, China.
Department of Reproductive Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
Clin Transl Oncol. 2025 May;27(5):2067-2083. doi: 10.1007/s12094-024-03713-z. Epub 2024 Oct 5.
Endometrial cancer (UCEC) is one of the most common malignant tumors in gynecology, and early diagnosis is crucial for its treatment. Currently, there is a lack of early screening tests specific to UCEC, and treatment advances are limited. It is crucial to identify more sensitive biomarkers for screening, diagnosis, and predicting UCEC. Previous studies have shown that UBE2T is involved in the development of various tumors such as breast cancer and liver cancer, but research on the role of UBE2T in UCEC is limited.
Using data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and UALCAN databases, we analyzed the differential expression of UBE2T mRNA and protein in endometrial cancer (UCEC), along with its clinical relevance. A total of 113 clinical samples were collected, and immunohistochemistry and Western blot analysis were employed to validate bioinformatics analysis results. Volcano plots were generated using UBE2T and its differentially expressed genes, and a protein-protein interaction (PPI) network was constructed. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), gene set enrichment analysis (GSEA), and immune infiltration analysis were used to predict the functional role of UBE2T in UCEC progression. Correlation between UBE2T expression and patient survival was analyzed using TCGA data, and Kaplan-Meier survival curves were plotted.
UBE2T is significantly overexpressed in UCEC and correlates with poor prognosis. Its overexpression is closely associated with mitosis, cell cycle regulation, and histological grade in UCEC patients.
UBE2T is highly expressed in UCEC and suppresses anti-tumor immune responses in UCEC patients. It serves as a key participant in UCEC progression, associated with a range of adverse outcomes, and holds potential as a clinical diagnostic and prognostic biomarker.
子宫内膜癌（UCEC）是妇科最常见的恶性肿瘤之一，早期诊断对其治疗至关重要。目前，缺乏针对UCEC的早期筛查检测方法，且治疗进展有限。识别更敏感的生物标志物用于UCEC的筛查、诊断和预测至关重要。先前的研究表明，UBE2T参与了乳腺癌和肝癌等多种肿瘤的发生发展，但关于UBE2T在UCEC中的作用研究有限。
利用来自癌症基因组图谱（TCGA）、基因表达综合数据库（GEO）和UALCAN数据库的数据，我们分析了UBE2T mRNA和蛋白在子宫内膜癌（UCEC）中的差异表达及其临床相关性。共收集了113份临床样本，并采用免疫组织化学和蛋白质印迹分析来验证生物信息学分析结果。使用UBE2T及其差异表达基因生成火山图，并构建蛋白质-蛋白质相互作用（PPI）网络。基因本体论（GO）、京都基因与基因组百科全书（KEGG）、基因集富集分析（GSEA）和免疫浸润分析用于预测UBE2T在UCEC进展中的功能作用。利用TCGA数据分析UBE2T表达与患者生存之间的相关性，并绘制Kaplan-Meier生存曲线。
UBE2T在UCEC中显著过表达，且与不良预后相关。其过表达与UCEC患者的有丝分裂、细胞周期调控和组织学分级密切相关。
UBE2T在UCEC中高表达，并抑制UCEC患者的抗肿瘤免疫反应。它是UCEC进展的关键参与者，与一系列不良结局相关，具有作为临床诊断和预后生物标志物的潜力。
