School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212100, People's Republic of China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
Mikrochim Acta. 2024 Oct 5;191(11):647. doi: 10.1007/s00604-024-06739-w.
Hydrogen peroxide-based Fenton reaction can effectively degrade many small-molecule fluorescent dyes, leading to notable alterations in fluorescence signals. Additionally, the two-dimensional black phosphorus/platinum nanocomposite (BP/Pt) demonstrates exceptional catalase (CAT) characteristics. Based on these, a colorimetric-fluorescence dual-mode signal output pattern based on BP/Pt-Fenton reaction-rhodamine B tandem reaction system is reported. The physical adsorption property of the BP/Pt nanozymes was utilized to couple with antibodies, thus constructing a novel dual-mode nanozyme-based immuno-sensing assay (NISA). By using the migratory antibiotic enrofloxacin (ENR) as the target, the NISA provided highly sensitive detection with the detection limits of 0.058 ng/mL for colorimetric-mode and 0.025 ng/mL for fluorescence-mode and achieved accurate quantitative detection in environmental water and crucian carp samples. This work provides an innovative design for monitoring antibiotics in the environment and broadens the idea for the application of nanozymes and Fenton systems in immunosensing assays.
基于过氧化氢的芬顿反应可以有效地降解许多小分子荧光染料，导致荧光信号发生显著变化。此外，二维黑磷/铂纳米复合材料（BP/Pt）表现出卓越的过氧化氢酶（CAT）特性。基于此，报道了一种基于 BP/Pt-Fenton 反应-罗丹明 B 串联反应体系的比色-荧光双模信号输出模式。BP/Pt 纳米酶的物理吸附特性被用于与抗体偶联，从而构建了一种新型的双模纳米酶基于免疫测定法（NISA）。以迁徙性抗生素恩诺沙星（ENR）为目标，该 NISA 提供了高度敏感的检测，比色模式的检测限为 0.058ng/mL，荧光模式的检测限为 0.025ng/mL，在环境水和鲫鱼样品中实现了准确的定量检测。这项工作为监测环境中的抗生素提供了一种创新的设计，并拓宽了纳米酶和芬顿系统在免疫测定中的应用思路。
