Li Shijie, Wen Wenjun, Guo Jianping, Wang Shuo, Wang Junping
Medical College, Nankai University, Tianjin 300500, China.
State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Food Chem. 2021 Sep 30;357:129766. doi: 10.1016/j.foodchem.2021.129766. Epub 2021 Apr 21.
The two-dimensional black phosphorus nanosheets (BPNSs) provide strong support for the construction of nanozymes with high catalytic performance due to the sheet structure and high electronic activity. A peroxidase-like BP-Pt nanocomposites was successfully synthesized using the instability of BPNS, a non-enzymatic immunosensing assay (NISA) was established with BP-Pt as immunosensing probe. Take the antibiotic enrofloxacin (ENR) as the target, NISA realized the highly sensitive ENR detection with detection limit (IC) of 0.005 μg/L. In addition, based on the good photothermal performance of oxTMB at 808 nm, a photothermal immunosensing assay (PT-NISA) was established, and ENR detection results was similar to NISA were obtained. In the analysis of the samples, the same detection results as the commercially available enzyme-linked immunoassay kit were obtained. These NISA and PT-NISA provide a more rapid and promising strategy for detecting food contaminants, and was expected to be used to detect other highly sensitive biological macromolecules.
二维黑磷纳米片（BPNSs）由于其片状结构和高电子活性，为构建具有高催化性能的纳米酶提供了有力支持。利用BPNS的不稳定性成功合成了一种类过氧化物酶的BP-Pt纳米复合材料，以BP-Pt作为免疫传感探针建立了非酶免疫传感分析（NISA）方法。以抗生素恩诺沙星（ENR）为目标物，NISA实现了对ENR的高灵敏检测，检测限（IC）为0.005 μg/L。此外，基于氧化态四甲基联苯胺（oxTMB）在808 nm处良好的光热性能，建立了光热免疫传感分析（PT-NISA）方法，获得了与NISA相似的ENR检测结果。在样品分析中，得到了与市售酶联免疫分析试剂盒相同的检测结果。这些NISA和PT-NISA为检测食品污染物提供了一种更快速且有前景的策略，有望用于检测其他高灵敏生物大分子。
