Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, 518120, Shenzhen, China.
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070, Wuhan, China.
Nat Commun. 2024 Oct 5;15(1):8652. doi: 10.1038/s41467-024-53044-4.
The genetic analysis of potato is hampered by the complexity of tetrasomic inheritance. An ongoing effort aims to transform the clonally propagated tetraploid potato into a seed-propagated diploid crop, which would make genetic analyses much easier owing to disomic inheritance. Here, we construct and report the large-scale genetic and heterotic characteristics of a diploid F potato population derived from the cross of two highly homozygous inbred lines. We investigate 20,382 traits generated from multi-omics dataset and identify 25,770 quantitative trait loci (QTLs). Coupled with gene expression data, we construct a systems-genetics network for gene discovery in potatoes. Importantly, we explore the genetic basis of heterosis in this population, especially for yield and male fertility heterosis. We find that positive heterotic effects of yield-related QTLs and negative heterotic effects of metabolite QTLs (mQTLs) contribute to yield heterosis. Additionally, we identify a PME gene with a dominance heterotic effect that plays an important role in male fertility heterosis. This study provides genetic resources for the potato community and will facilitate the application of heterosis in diploid potato breeding.
马铃薯的遗传分析受到四倍体遗传复杂性的阻碍。目前正在努力将克隆繁殖的四倍体马铃薯转化为种子繁殖的二倍体作物，这将由于二倍体遗传而使遗传分析变得更加容易。在这里，我们构建并报告了来自两个高度纯合自交系杂交的二倍体 F 马铃薯群体的大规模遗传和杂种优势特征。我们研究了来自多组学数据集的 20,382 个特征，并鉴定了 25,770 个数量性状位点 (QTL)。结合基因表达数据，我们构建了一个用于马铃薯中基因发现的系统遗传学网络。重要的是，我们探索了该群体杂种优势的遗传基础，特别是产量和雄性育性杂种优势。我们发现，与产量相关的 QTL 的正向杂种优势效应和代谢物 QTL（mQTL）的负向杂种优势效应有助于产量杂种优势。此外，我们鉴定了一个具有显性杂种优势效应的 PME 基因，该基因在雄性育性杂种优势中起重要作用。这项研究为马铃薯研究界提供了遗传资源，并将促进杂种优势在二倍体马铃薯育种中的应用。
