Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization & College of Oceanography, Hohai University, Nanjing, People's Republic of China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Curr Microbiol. 2024 Oct 6;81(11):392. doi: 10.1007/s00284-024-03922-3.
A Gram-staining negative, non-motile, rod-shaped, oxidase negative and catalase positive strain WL0021 was isolated from cricket (Gryllus chinensis) living in the campus of Hohai University. Strain WL0021 was characterized utilizing a polyphasic taxonomy approach. The major fatty acids (> 5%) for strain WL0021 were C and summed feature 8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phospholipid, two aminolipids, and an unidentified polar lipid. Ubiquinone-10 was detected as the predominant respiratory quinone. The results of 16S rRNA gene phylogenetic analyses revealed that strain WL0021 had the highest sequence similarity of 95.3% to Microvirga flavescens c27j1 and strain WL0021 formed a distinct linage within the family Methylobacteriaceae in the phylogenetic trees. Whole genomic DNA G+C content was 48.3%. Combined with the results from this study, strain WL0021 should represent a novel genus in the family Methylobacteriaceae, for which the name Hohaiivirga grylli gen. nov., sp. nov. (type strain WL0021=GDMCC 1.2420 =JCM 34655=MCCC 1K05886) is proposed.
从生活在河海大学校园里的蟋蟀（Gryllus chinensis）中分离到一株革兰氏染色阴性、非运动、杆状、氧化酶阴性和过氧化氢酶阳性的菌株 WL0021。利用多相分类学方法对菌株 WL0021 进行了表征。菌株 WL0021 的主要脂肪酸（>5%）为 C 和特征脂肪酸 8，主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、磷脂、两种氨基脂和一种未鉴定的极性脂。检测到泛醌-10 作为主要的呼吸醌。16S rRNA 基因系统发育分析的结果表明，菌株 WL0021 与 Microvirga flavescens c27j1 的序列相似性最高，为 95.3%，菌株 WL0021 在系统发育树中与 Methylobacteriaceae 家族中的其他菌株形成了一个独特的分支。全基因组 DNA G+C 含量为 48.3%。结合本研究结果，菌株 WL0021 应代表 Methylobacteriaceae 家族中的一个新属，因此建议将其命名为 Hohaiivirga grylli gen. nov., sp. nov.（模式菌株 WL0021=GDMCC 1.2420=JCM 34655=MCCC 1K05886）。
