Department of Turf Science and engineering, College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Department of Turf Science and engineering, College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
J Proteomics. 2025 Jan 6;310:105325. doi: 10.1016/j.jprot.2024.105325. Epub 2024 Oct 5.
Dehydration priming (DP) induces stress memory which plays a positive role in plant adaptability, but it is not well understood how DP differentially regulates subsequent dehydration (cis priming) or salt (trans priming) tolerance at the post-translational level. Purpose of this study was to identify proteins, phosphorylation levels and sites, and relevant metabolic pathways for DP-induced dehydration or salt tolerance in Agrostis stolonifera. DP-induced differentially regulated proteins (DRPs) were mostly located in the cytoplasm, chloroplast, and cell membrane, and differentially regulated phosphoproteins (DRPPs) were mostly nuclear proteins and cytoplasmic proteins. DP regulated common phosphorylation sites ([SP] and [RxxS]) under dehydration and salt conditions and also individually affected 8 or 11 phosphorylation sites under dehydration or salt stress. DP-regulated DRPPs were mainly rich in glycolysis and glutathione metabolism pathways, RNA splicing, and dynamin family proteins under dehydration stress, whereas DP-regulated salt tolerance was mainly related to chlorophyll metabolism, photosynthesis, MAPK signaling cascade, and ABC transporter I family at the phosphorylation level. In addition, the DP also significantly up-regulated phosphorylation of histones (ATXR3 and SETD1A) in response to subsequent dehydration and salt stress as well as abundances of antioxidant enzymes, dynamin family protein, and KCS6 under dehydration stress or abundances of PETE, HMGA, XTH, and ABCI6 under salt stress, respectively. Transcriptomics analysis further indicated that DP-regulated dehydration or salt tolerance was also related to transcriptional regulation in the early stage. Current results provided better understanding of the role of stress memory in plant adaptability to repeated or crossed stress via post-translational modifications (PTMs). SIGNIFICANCE: Recurrent moderate drought may buffer drought legacies in many plant species. When plants were exposed to repeated drought stress, their adaptability to subsequent stress could be enhanced, which is known as "stress memory". Dehydration priming has been found to be an important approach to induce stress memory. Current results provided better understanding of the role of stress memory in plant adaptability to repeated or crossed stress via post-translational modifications.
脱水预适应（DP）诱导应激记忆，在植物适应能力方面发挥积极作用，但 DP 如何在后翻译水平上差异化调节随后的脱水（顺式预适应）或盐（反式预适应）耐受性尚不清楚。本研究的目的是鉴定 DP 诱导匍匐翦股颖脱水或耐盐性的差异调节蛋白（DRPs）、磷酸化水平和位点，以及相关代谢途径。DP 诱导的差异调节蛋白（DRPs）主要位于细胞质、叶绿体和细胞膜中，差异调节磷酸化蛋白（DRPPs）主要是核蛋白和细胞质蛋白。DP 调节脱水和盐胁迫下的共同磷酸化位点（[SP]和[RxxS]），并分别单独影响脱水或盐胁迫下的 8 或 11 个磷酸化位点。DP 调节的 DRPPs 在脱水胁迫下主要富含糖酵解和谷胱甘肽代谢途径、RNA 剪接和动力蛋白家族蛋白，而 DP 调节的耐盐性主要与叶绿素代谢、光合作用、MAPK 信号级联和 ABC 转运体 I 家族在磷酸化水平上相关。此外，DP 还显著上调了随后脱水和盐胁迫下组蛋白（ATXR3 和 SETD1A）的磷酸化以及抗氧化酶、动力蛋白家族蛋白和 KCS6 的丰度在脱水胁迫下或 PETE、HMGA、XTH 和 ABCI6 的丰度在盐胁迫下。转录组学分析进一步表明，DP 调节的脱水或耐盐性也与早期的转录调控有关。目前的研究结果为通过翻译后修饰（PTMs）理解应激记忆在植物对重复或交叉胁迫的适应性中的作用提供了更好的认识。意义：反复出现的中度干旱可能会缓冲许多植物物种中的干旱遗留问题。当植物暴露于重复的干旱胁迫下时，它们对后续胁迫的适应能力可以增强，这被称为“应激记忆”。脱水预适应已被发现是诱导应激记忆的一种重要方法。目前的研究结果为通过翻译后修饰理解应激记忆在植物对重复或交叉胁迫的适应性中的作用提供了更好的认识。
