甜樱桃（Prunus cerasus L.）中抗性基因类似物的研究进展。

A landscape of resistance gene analogs in sour cherry (Prunus cerasus L.).

机构信息

Institute for Breeding Research on Fruit Crops, Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Dresden, 01326, Saxony, Germany.

出版信息

BMC Res Notes. 2024 Oct 6;17(1):292. doi: 10.1186/s13104-024-06952-z.

DOI:10.1186/s13104-024-06952-z

PMID:39370523

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11457318/

Abstract

OBJECTIVE

This research aims to analyze the presence and distribution of resistance genes in the avium and fruticosa subgenomes of Prunus cerasus through computational methods and bioinformatics tools.

RESULTS

Analysis of genome and transcriptome sequencing data revealed a total of 19,570 transcripts with at least one resistance gene domain in Prunus cerasus subgenome avium and 19,142 in Prunus cerasus subgenome fruticosa. Key findings include the identification of 804 "complete" resistance gene transcripts in Prunus cerasus subgenome avium and 817 in Prunus cerasus subgenome fruticosa, with distinct distributions of resistance gene classes observed between the subgenomes. Phylogenetic analysis showed clustering of resistance genes, and unique resistance proteins were identified in each subgenome. Functional annotation comparisons with Arabidopsis thaliana highlighted shared and unique resistance genes, emphasizing the complexity of disease resistance in cherry species. Additionally, a higher diversity of RLKs and RLPs was observed, with 504 transcripts identified and 18 showing similarity to known reference genes.

摘要

目的

本研究旨在通过计算方法和生物信息学工具分析甜樱桃的 avium 和 fruticosa 亚基因组中抗性基因的存在和分布。

结果

对基因组和转录组测序数据的分析显示，在甜樱桃 avium 亚基因组中共有 19570 个转录本至少具有一个抗性基因结构域，在甜樱桃 fruticosa 亚基因组中则有 19142 个。主要发现包括在甜樱桃 avium 亚基因组中鉴定出 804 个“完整”抗性基因转录本，在甜樱桃 fruticosa 亚基因组中鉴定出 817 个，两个亚基因组中抗性基因类别的分布存在明显差异。系统发育分析显示抗性基因聚类，每个亚基因组中都鉴定出独特的抗性蛋白。与拟南芥的功能注释比较突出了共享和独特的抗性基因，强调了樱桃属植物抗病性的复杂性。此外，还观察到 RLKs 和 RLPs 的多样性更高，共鉴定出 504 个转录本，其中 18 个与已知参考基因具有相似性。