Yang Jing, Sun Ning, Wang Wenru, Zhang Ruihua, Sun Siqi, Li Biqi, Shi Yue, Zeng Junfeng, Jia Shulei
School of Basic Medicine, Shanxi Medical University, Taiyuan, Shanxi, China.
Department of Psychiatry, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Front Genet. 2024 Sep 20;15:1469600. doi: 10.3389/fgene.2024.1469600. eCollection 2024.
Pullulan is a kind of natural polymer, which is widely used in medicine and food because of its solubility, plasticity, edible, non-toxicity and good biocompatibility. It is of great significance to improve the yield of pullulan by genetic modification of microorganisms. It was previously reported that TN3-1 isolated from honey-comb could produce high-yield of pullulan, but the molecular mechanisms of its production of pullulan had not been completely solved. In this study, the reported strains of spp. were further compared and analyzed at genome level. It was found that genome duplication and genome genetic variations might be the crucial factors for the high yield of pullulan and stress resistance. This particular phenotype may be the result of adaptive evolution, which can adapt to its environment through genetic variation and adaptive selection. In addition, the TN3-1 strain has a large genome, and the special regulatory sequences of its specific genes and promoters may ensure a unique characteristics. This study is a supplement of the previous studies, and provides basic data for the research of microbial genome modification in food and healthcare applications.
普鲁兰多糖是一种天然聚合物，因其具有溶解性、可塑性、可食用、无毒且生物相容性良好等特点，而被广泛应用于医药和食品领域。通过对微生物进行基因改造来提高普鲁兰多糖的产量具有重要意义。此前有报道称，从蜂巢中分离出的TN3-1菌株能够高产普鲁兰多糖，但其产生普鲁兰多糖的分子机制尚未完全解决。在本研究中，对已报道的菌株在基因组水平上进行了进一步的比较和分析。研究发现，基因组复制和基因组遗传变异可能是普鲁兰多糖高产和抗逆性的关键因素。这种特殊的表型可能是适应性进化的结果，它可以通过遗传变异和适应性选择来适应其环境。此外，TN3-1菌株具有较大的基因组，其特定基因和启动子的特殊调控序列可能确保了其独特的特性。本研究是对先前研究的补充，并为食品和医疗保健应用中微生物基因组改造的研究提供了基础数据。
