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高中课堂中的噬菌体搜寻:噬菌体的分离与鉴定

Phage Hunting in the High School Classroom: Phage Isolation and Characterization.

作者信息

Bittle Charles, Brittain Kathryn, Doore Sarah M, Dover John, Drarvik Silje M Bergland, Parent Kristin N, Ratnayake Rochelle, Stone Peter, Schrad Jason R, Schrad Kevin, Subramanian Sundharraman, Tinney Kendal R

机构信息

Lincoln Southwest High School.

assisted as part of the instructional team at Michigan State University in the Department of Cell and Molecular Biology.

出版信息

Am Biol Teach. 2023 Oct;85(8):440-447. doi: 10.1525/abt.2023.85.8.440.

Abstract

National science, technology, engineering, and mathematics education emphasizes science practices, such as hands-on learning. We describe a week-long activity where students participate in real-world scientific discovery, including "hunting" for bacteriophage in a variety of environmental samples. First, the students collect samples, then look for evidence of phage on "bait" bacteria, and finally amplify/purify the phages for further study.

摘要

国家科学、技术、工程和数学教育强调科学实践,例如动手学习。我们描述了一项为期一周的活动,学生们在其中参与现实世界的科学探索,包括在各种环境样本中“搜寻”噬菌体。首先,学生们收集样本,然后在“诱饵”细菌上寻找噬菌体的证据,最后扩增/纯化噬菌体以供进一步研究。

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