Geogi Chris Cherian, Dubey Sandeep, Singh Palak, Rajkumar B, Samant Praveen Singh, Rawat Ananya
Dept. of Conservative Dentistry and Endodontics, Babu Banarasi Das College of Dental Sciences, Lucknow (U.P.), India.
J Dent (Shiraz). 2024 Sep 1;25(3):236-242. doi: 10.30476/dentjods.2023.98298.2071. eCollection 2024 Sep.
During endodontic therapy, irrigation solutions applied in the root canal may affect the physicochemical properties of the dentinal wall, thereby changing its microhardness. This may adversely affect the sealing ability and adhesion of dental materials. Therefore, many studies have focused on the search for an ideal root canal irrigant that has a minimal effect on dentinal microhardness.
This study was conducted to determine the changes in dentin microhardness after root canal irrigation with different endodontic irrigants.
Ninety-five freshly extracted maxillary central incisor teeth with straight single canals were selected. These teeth were sectioned transversely at the level of the cementoenamel junction. The working length of each tooth was determined, and canal space was prepared by the HyFlex CM rotary file system. During instrumentation, normal saline was used for irrigation. Then, teeth were split longitudinally into two segments. According to the irrigating solution employed, samples were divided into five groups (n=19): normal saline (Group A), 3% sodium hypochlorite (Group B), 2% chlorhexidine (Group C), 5% calcium hypochlorite (Group D), and 0.2% nanochitosan (Group E).3 mL of the corresponding irrigating solution was administered for total15 minutes in each prepared sample. The Vickers micro-hardness tester was then used to assess micro-hardness. The data was analyzed using one-way analysis of variance (ANOVA).
All tested irrigating solutions decreased the dentinal microhardness. Samples irrigated with 5% calcium hypochlorite demonstrated dentinal microhardness of 42.43±1.62, which is the lowest among all the tested groups, followed by nano chitosan, sodium hypochlorite, and chlorhexidine. Samples treated with control group (saline) demonstrated the maximum microhardness of dentin in the present study.
Within the limitations of this research, it can be concluded that the tested novel irrigating solutions, 5% calcium hypochlorite and 0.2% nanochitosan, were more detrimental to radicular dentin microhardness when compared with conventional endodontic irrigants.
在根管治疗过程中，应用于根管的冲洗液可能会影响牙本质壁的物理化学性质，从而改变其显微硬度。这可能会对牙科材料的封闭能力和黏附性产生不利影响。因此，许多研究都集中在寻找一种对牙本质显微硬度影响最小的理想根管冲洗剂上。
本研究旨在确定使用不同根管冲洗剂进行根管冲洗后牙本质显微硬度的变化。
选取95颗新鲜拔除的上颌中切牙，根管为直单根管。这些牙齿在牙骨质釉质界水平横向切开。确定每颗牙齿的工作长度，并用HyFlex CM旋转锉系统预备根管空间。在预备过程中，用生理盐水冲洗。然后，将牙齿纵向劈成两段。根据所使用的冲洗液，样本分为五组（n = 19）：生理盐水（A组）、3%次氯酸钠（B组）、2%氯己定（C组）、5%次氯酸钙（D组）和0.2%纳米壳聚糖（E组）。在每个预备好的样本中注入3 mL相应的冲洗液，共15分钟。然后使用维氏显微硬度测试仪评估显微硬度。数据采用单因素方差分析（ANOVA）进行分析。
所有测试的冲洗液均降低了牙本质显微硬度。用5%次氯酸钙冲洗的样本显示牙本质显微硬度为42.43±1.62，是所有测试组中最低的，其次是纳米壳聚糖、次氯酸钠和氯己定。在本研究中，用对照组（生理盐水）处理的样本显示牙本质的显微硬度最高。
在本研究的局限性内，可以得出结论，与传统的根管冲洗剂相比，所测试的新型冲洗液5%次氯酸钙和0.2%纳米壳聚糖对根管牙本质显微硬度的损害更大。
